Invitrogen

MASH1 Monoclonal Antibody (24B72D11), eBioscience™

MASH1 Antibody in Immunocytochemistry (ICC/IF)

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MASH1 Antibody (14-5794-82) in ICC/IF

Immunocytochemistry of PC-12 cells stained with 20 µg/mL Mouse IgG1 Isotype Control (left) or 20 µg/mL Anti-Mouse/Rat MASH1 Purified (right) followed by Anti-Mouse IgG TRITC.

MASH1 Antibody in Immunohistochemistry (IHC)

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产品信息

14-5794-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5 µg/mL

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免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

20 µg/mL

产品规格

种属反应

Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

24B72D11

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2572887

产品详细信息

Description: The monoclonal antibody 24B72D11 recognizes mouse and rat Mammalian achaete-scute homolog 1 (MASH1), a 34 kDa basic helix-loop-helix (bHLH) transcription factor. MASH1, like family members Olig2 and Ngn2, is expressed in progenitor cells throughout the nervous system and retina and is a key regulator of cell fate specification. Expression of MASH1 is regulated by the Delta/Notch1 pathway through direct binding of Hes1 to MASH1 as well as competitive binding to MASH1 hetero-dimerizing proteins. At a transcriptional level, MASH1 expression is controlled by notch-mediated regulation of sox2, which binds to the MASH1 promoter region up-regulating expression. MASH1 has been implicated in the formation of multiple neural cell types including dopaminergic, serotonergic, glutaminergic and GABAergic neurons. Overexpression of MASH1 has been shown in small cell lung carcinoma and medullary thyroid cancer.

Applications Reported: This 24B72D11 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, and immunocytochemistry.

Applications Tested: This 24B72D11 antibody has been tested by western blot on reduced PC12 cells and can be used at less than or equal to 5 µg/mL. The 24B72D11 antibody has also been tested by immunocytochemistry on fixed and permeablized PC12 cells at can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ASCL1 (also known as ASH1) is a basic helix-loop-helix transcription factor that is required for early development of the nervous system. Expressed in fetal brain, ASCL1 is essential for the proper development of autonomic neurons and for the survival of subsets of autonomic neurons. ASCL1 interaction with MEF-2A may regulate the expression of specific genes that are critical for the formation of distinct neuronal circuits within the central nervous system. The high level of ASCL1 expression in neuroendocrine tumors, such as medullary thyroid cancer, small cell lung cancer and lung cancer with neuroendocrine features may provide a useful marker for cancers with neuroendocrine features. Mapping to human chromosome 12, the ASCL1 gene contains a trinucleotide repeat region, making this locus a candidate for inherited disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Treatment with Amniotic Fluid Stem Cell Extracellular Vesicles Promotes Fetal Lung Branching and Cell Differentiation at Canalicular and Saccular Stages in Experimental Pulmonary Hypoplasia Secondary to Congenital Diaphragmatic Hernia.

Stem cells translational medicine

Khalaj K,Figueira RL,Antounians L,Gandhi S,Wales M,Montalva L,Biouss G,Zani A

14-5794-82 was used in Western Blotting and Immunohistochemistry to suggest that AFSC-EV-based therapies hold potential for restoring fetal lung growth and maturation in babies with pulmonary hypoplasia secondary to CDH.

Fri Oct 21 00:00:00 UTC 2022

Stem cells translational medicine

Khalaj K,Figueira RL,Antounians L,Gandhi S,Wales M,Montalva L,Biouss G,Zani A

Fri Oct 21 00:00:00 UTC 2022

Neurogenic Potential of the 18-kDa Mitochondrial Translocator Protein (TSPO) in Pluripotent P19 Stem Cells.

Cells

González-Blanco L,Bermejo-Millo JC,Oliveira G,Potes Y,Antuña E,Menéndez-Valle I,Vega-Naredo I,Coto-Montes A,Caballero B

14-5794-82 was used in Western Blotting to study changes in cell morphology, cell proliferation, cell death, the cell cycle, mitochondrial functionality, and the levels of pluripotency and neurogenesis of P19 stem cells treated with the TSPO ligand, PK 11195, in comparison to differentiation induced by retinoid acid (RA) and undifferentiated P19 stem cells.

Sun Oct 17 00:00:00 UTC 2021

Neurogenic Potential of the 18-kDa Mitochondrial Translocator Protein (TSPO) in Pluripotent P19 Stem Cells.

Cells

González-Blanco L,Bermejo-Millo JC,Oliveira G,Potes Y,Antuña E,Menéndez-Valle I,Vega-Naredo I,Coto-Montes A,Caballero B

Sun Oct 17 00:00:00 UTC 2021

Jagged and Delta-like ligands control distinct events during airway progenitor cell differentiation.

eLife

Stupnikov MR,Yang Y,Mori M,Lu J,Cardoso WV

14-5794 was used in Immunohistochemistry-immunofluorescence to determine how Jagged or Delta-like ligands influence differentiation of airway epithelial progenitors.

Mon Oct 21 00:00:00 UTC 2019

Jagged and Delta-like ligands control distinct events during airway progenitor cell differentiation.

eLife

Stupnikov MR,Yang Y,Mori M,Lu J,Cardoso WV

14-5794 was used in Immunohistochemistry-immunofluorescence to determine how Jagged or Delta-like ligands influence differentiation of airway epithelial progenitors.

Mon Oct 21 00:00:00 UTC 2019

Neural stem cells sustain natural killer cells that dictate recovery from brain inflammation.

Nature neuroscience

Liu Q,Sanai N,Jin WN,La Cava A,Van Kaer L,Shi FD

14-5794 was used in Immunohistochemistry to reveal that reciprocal interactions between neural stem cells and natural killer cells regulate neurorepair.

Mon Feb 01 00:00:00 UTC 2016

生物信息学

蛋白别名: achaete-scute complex homolog 1; achaete-scute complex homolog-like 1; achaete-scute complex-like protein 1; Achaete-scute homolog 1; ASH-1; KMT2H; mammalian achaete scute homolog 1; mASH-1

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基因别名: AI225900; Ascl1; ASH1; bHLHa46; Mash-1; Mash1

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UniProt ID: (Rat) P19359, (Mouse) Q02067

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Entrez Gene ID: (Rat) 64186, (Mouse) 17172

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功能