Invitrogen

MBD2 Polyclonal Antibody

MBD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MBD2 Antibody (PA1-29386) in IHC (P)

Immunohistochemistry (Paraffin) analysis of MBD2 in human heart tissue using MBD2 Polyclonal Antibody (Product # PA1-29386) at a dilution of 1:100. Left to right:DAPI, MBD2, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.

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产品信息

PA1-29386

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-2.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-10 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to human MBD2 (NP_056647)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2139741

产品详细信息

Recommended positive controls: HepG2, mouse ES cells.

Predicted reactivity: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%.

靶标信息

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。