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Product Details
PA5-18619
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (RNDPLNQNKGKPDLN) corresponding to the internal amino acids of MBD2 (aa 226-241).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_10977203
Product Specific Information
This antibody is predicted to react with canine, mouse and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Demethylase; DKFZp586O0821; DMTase; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2
Gene Aliases: DMTase; MBD2; NY-CO-41
UniProt ID: (Human) Q9UBB5
Entrez Gene ID: (Human) 8932
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
satellite DNA binding
mRNA binding
protein binding
methyl-CpG binding
protein domain specific binding
nucleosomal DNA binding
siRNA binding
C2H2 zinc finger domain binding
negative regulation of transcription from RNA polymerase II promoter
chromatin silencing at rDNA
methylation-dependent chromatin silencing
transcription, DNA-templated
positive regulation of Wnt signaling pathway
regulation of cell proliferation
maternal behavior
ATP-dependent chromatin remodeling
cellular protein complex assembly
negative regulation of transcription, DNA-templated
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