Invitrogen

MBD2 Recombinant Rabbit Monoclonal Antibody (JE57-73)

MBD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MBD2 Antibody (MA5-42520) in IHC (P)

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human breast carcinoma tissue using MBD2 Recombinant Monoclonal Antibody (Product # MA5-42520). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the MBD2 antibo... View More

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产品信息

MA5-42520

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE57-73

抗原

Recombinant protein within human MBD2 aa 50-200

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2911661

产品详细信息

Positive Control: Raji cell lysates, rat kidney tissue, human lung carcinoma tissue, human breast carcinoma tissue, Daudi.

Subcellular Location: Nucleus.

靶标信息

Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。