Invitrogen

MBD3 Recombinant Rabbit Monoclonal Antibody (9X1J7)

MBD3 Antibody in Immunocytochemistry (ICC/IF)

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MBD3 Antibody (MA5-35733) in ICC/IF

Immunofluorescence analysis of Methyl-CpG-binding protein MBD3 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MBD3 Recombinant Rabbit Monoclonal Antibody (ARC1349) (Product # MA5-35733) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Se... View More

MBD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MBD3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with Methyl-CpG-binding protein MBD3 siRNA and decrease in signal intensity was observed in Western Blot application using MBD3 Recombinant Rabbit Monoclonal Antibody (ARC1349) (Product # MA5-35733). {KD}

产品信息

MA5-35733

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

9X1J7

抗原

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MBD3 (O95983)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2849633

产品详细信息

Immunogen sequence: MERKRWECPA LPQGWEREEV PRRSGLSAGH RDVFYYSPSG KKFRSKPQLA RYLGGSMDLS TFDFRTGKML MSKMNKSRQR VRYDSSNQVK GKPDLNTALP

靶标信息

Regulator of genomic integrity and cellular response to DNA damage acting through ATR-mediated checkpoint signaling. Necessary for centrosome duplication. It functions as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, two molecules involved in centriole formation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。