Invitrogen

MBD3 Recombinant Rabbit Monoclonal Antibody (JB61-47)

MBD3 Antibody in Immunocytochemistry (ICC/IF)

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MBD3 Antibody (MA5-34711) in ICC/IF

Immunofluorescence analysis of Methyl-CpG-binding protein MBD3 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MBD3 Recombinant Rabbit Monoclonal Antibody (JB61-47) (Product # MA5-34711) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Se... View More

MBD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MBD3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with Methyl-CpG-binding protein MBD3 siRNA and decrease in signal intensity was observed in Western Blot application using MBD3 Recombinant Rabbit Monoclonal Antibody (JB61-47) (Product # MA5-34711). {KD}

产品信息

MA5-34711

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JB61-47

抗原

Recombinant protein within Human MBD3 aa 160-291

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Wet ice

AB_2848619

产品详细信息

Positive Control: U937, A549, NIH-3T3, SH-SY-5Y, rat testis tissue, human placenta tissue, mouse brain tissue.

靶标信息

Regulator of genomic integrity and cellular response to DNA damage acting through ATR-mediated checkpoint signaling. Necessary for centrosome duplication. It functions as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, two molecules involved in centriole formation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。