Invitrogen

MBL2 Monoclonal Antibody (3E7)

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MBL2 Antibody (MA1-40145) in Flow

Science advances 2023 - Fig. 2. MBL and hItln1 binding to microbial populations in human stool samples. ( A ) Schematic of flow cytometry-based assay to measure soluble lectin binding to stool microbiome. ( B ) Flow cytometry analysis of healthy human stool plotted as SYTO BC against lectin binding (anti-Strep DY649). Panels show no lectin control (column 1), and strep-MBL (top row), or strep-hItln1 (bottom row) binding in the pre... View More

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产品信息

MA1-40145

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

1:50

流式细胞分析 (Flow)

1:50

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酶联免疫吸附实验 (ELISA)

1:50

功能检测 (Functional)

1:50

in situ PLA (PLA)

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产品规格

种属反应

Human

已发表种属

Human, Rhesus monkey, Yeast

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3E7

抗原

Serum isolated human RaRF

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Protein G

保存液

PBS with 0.1% BSA

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_1076469

产品详细信息

MA1-40145 detects MBL from human samples.

The MA1-40145 immunogen is Mannose Binding Lectin (MBL).

MA1-40145 has been successfully used in flow cytometry, Western blot, ELISA, Functional Assays, and immunohistochemistry (frozen) applications.

Negative control: Nonimmune normal serum or TBS.

Endotoxin <24 EU/mg.

靶标信息

MBL2 is a calcium-dependent lectin involved in innate immune defense. It binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. It also binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. MBL2 may bind DNA.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Lectin-Seq: A method to profile lectin-microbe interactions in native communities.

Science advances

McPherson RL,Isabella CR,Walker RL,Sergio D,Bae S,Gaca T,Raman S,Nguyen LTT,Wesener DA,Halim M,Wuo MG,Dugan A,Kerby R,Ghosh S,Rey FE,Dhennezel C,Pishchany G,Lensch V,Vlamakis H,Alm EJ,Xavier RJ,Kiessling LL

Published figure using MBL2 monoclonal antibody (Product # MA1-40145) in Flow Cytometry

Fri Jul 28 00:00:00 UTC 2023

The classical pathway triggers pathogenic complement activation in membranous nephropathy.

Nature communications

Seifert L,Zahner G,Meyer-Schwesinger C,Hickstein N,Dehde S,Wulf S,Köllner SMS,Lucas R,Kylies D,Froembling S,Zielinski S,Kretz O,Borodovsky A,Biniaminov S,Wang Y,Cheng H,Koch-Nolte F,Zipfel PF,Hopfer H,Puelles VG,Panzer U,Huber TB,Wiech T,Tomas NM

MA1-40145 was used in Proximity Ligation Assay (PLA) to show that, in MN, complement is primarily activated via the classical pathway and targeting complement components such as C3 may represent a promising therapeutic strategy.

Sat Jan 28 00:00:00 UTC 2023

Rapid Transduction and Expansion of Transduced T Cells with Maintenance of Central Memory Populations.

Molecular therapy. Methods & clinical development

Pampusch MS,Haran KP,Hart GT,Rakasz EG,Rendahl AK,Berger EA,Connick E,Skinner PJ

Published figure using MBL2 monoclonal antibody (Product # MA1-40145) in Flow Cytometry

Fri Mar 13 00:00:00 UTC 2020

Simian Immunodeficiency Virus (SIV)-Specific Chimeric Antigen Receptor-T Cells Engineered to Target B Cell Follicles and Suppress SIV Replication.

Frontiers in immunology

Haran KP,Hajduczki A,Pampusch MS,Mwakalundwa G,Vargas-Inchaustegui DA,Rakasz EG,Connick E,Berger EA,Skinner PJ

MA1-40145 was used in Flow cytometry/Cell sorting to show that antiviral CAR/CXCR5-expressing T cells when infused into an SIV-infected animal or an HIV-infected individual, will home to B cell follicles suppress viral replication and lead to long-term durable remission of SIV and HIV.

Wed Apr 10 00:00:00 UTC 2019

Commercially available complement component-depleted sera are unexpectedly codepleted of ficolin-2.

Clinical and vaccine immunology : CVI

Brady AM,Geno KA,Dalecki AG,Cheng X,Nahm MH

Published figure using MBL2 monoclonal antibody (Product # MA1-40145) in Flow Cytometry

Mon Sep 01 00:00:00 UTC 2014

Blood collection tubes influence serum ficolin-1 and ficolin-2 levels.

Clinical and vaccine immunology : CVI

Brady AM,Spencer BL,Falsey AR,Nahm MH

MA1-40145 was used in flow cytometry to study the effect of different blood collection tubes on the measured levels of serum ficolin-1 and -2

Wed Jan 01 00:00:00 UTC 2014

生物信息学

蛋白别名: Collectin-1; Collectin1; mannan-binding lectin; Mannan-binding protein; Mannose-binding lectin; mannose-binding lectin (protein C) 2, soluble (opsonic defect); mannose-binding lectin 2, soluble (opsonic defect); Mannose-binding protein C; MBP-C; MBP1; MBPC; MGC116832; MGC116833

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基因别名: COLEC1; HSMBPC; MBL; MBL2; MBL2D; MBP; MBP-C; MBP1; MBPD

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UniProt ID: (Human) P11226

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Entrez Gene ID: (Human) 4153

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功能

serine-type endopeptidase activity receptor binding calcium ion binding protein binding mannose binding calcium-dependent protein binding

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