Invitrogen

MBP Monoclonal Antibody (MBP101)

MBP Antibody in Immunocytochemistry (ICC/IF)

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MBP Antibody (MA1-10837) in ICC/IF

Immunofluorescence analysis of MBP was performed using 70% confluent log phase RSC96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MBP Monoclonal Antibody (Product # MA1-10837, 1.6 µg/mL) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32766, 1:2,000), ... View More

MBP Antibody in Immunohistochemistry (IHC)

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MBP Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using MBP Monoclonal Antibody (Product # MA1-10837), shows increased expression in RSC96 cell line versus no expression in L6 cell line. {RE}

产品信息

MA1-10837

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-10 µg/mL

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免疫组化 (IHC)

1-10 µg/mL

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免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

1:64000

产品规格

种属反应

Goat, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat

已发表种属

Human, Marsupial, Rat, Rhesus monkey

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

MBP101

抗原

Purified human myelin basic protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_1077025

产品详细信息

MA1-10837 detects Myelin Basic Protein from human, non-human primate, rabbit, sheep, goat, rat, and mouse samples.

MA1-10837 has been successfully used in ELISA, Western blot, and immunohistochemistry applications.

The MA1-10837 immunogen is purified human myelin basic protein.

靶标信息

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Eosinophil and mast cell-derived exosomes promote integrity of intestinal mucosa via the NEAT1/miR-211-5p/glial cell line-derived neurotrophic factor axis in duodenum.

Environmental toxicology

Wang J,Gu S,Qin B

MA1-10837 was used in Immunohistochemistry to identify differential lncRNAs that were consistently and significantly up regulated in FD cases.

Wed Nov 01 00:00:00 UTC 2023

Neuronal and Glial Distribution of Tau Protein in the Adult Rat and Monkey.

Frontiers in molecular neuroscience

Kanaan NM,Grabinski T

Published figure using MBP monoclonal antibody (Product # MA1-10837) in Immunocytochemistry

Sat Aug 10 00:00:00 UTC 2024

Neuronal and Glial Distribution of Tau Protein in the Adult Rat and Monkey.

Frontiers in molecular neuroscience

Kanaan NM,Grabinski T

Published figure using MBP monoclonal antibody (Product # MA1-10837) in Immunocytochemistry

Sat Aug 10 00:00:00 UTC 2024

Neuronal and Glial Distribution of Tau Protein in the Adult Rat and Monkey.

Frontiers in molecular neuroscience

Kanaan NM,Grabinski T

Published figure using MBP monoclonal antibody (Product # MA1-10837) in Immunocytochemistry

Sat Aug 10 00:00:00 UTC 2024

BCAS1-positive immature oligodendrocytes are affected by the α-synuclein-induced pathology of multiple system atrophy.

Acta neuropathologica communications

Kaji S,Maki T,Ueda J,Ishimoto T,Inoue Y,Yasuda K,Sawamura M,Hikawa R,Ayaki T,Yamakado H,Takahashi R

Published figure using MBP monoclonal antibody (Product # MA1-10837) in Immunohistochemistry

Wed Jul 29 00:00:00 UTC 2020

BCAS1-positive immature oligodendrocytes are affected by the α-synuclein-induced pathology of multiple system atrophy.

Acta neuropathologica communications

Kaji S,Maki T,Ueda J,Ishimoto T,Inoue Y,Yasuda K,Sawamura M,Hikawa R,Ayaki T,Yamakado H,Takahashi R

Published figure using MBP monoclonal antibody (Product # MA1-10837) in Immunohistochemistry

Wed Jul 29 00:00:00 UTC 2020

BCAS1-positive immature oligodendrocytes are affected by the α-synuclein-induced pathology of multiple system atrophy.

Acta neuropathologica communications

Kaji S,Maki T,Ueda J,Ishimoto T,Inoue Y,Yasuda K,Sawamura M,Hikawa R,Ayaki T,Yamakado H,Takahashi R

Published figure using MBP monoclonal antibody (Product # MA1-10837) in Immunohistochemistry

Wed Jul 29 00:00:00 UTC 2020

KUS121, a valosin-containing protein modulator, attenuates ischemic stroke via preventing ATP depletion.

Scientific reports

Kinoshita H,Maki T,Yasuda K,Kishida N,Sasaoka N,Takagi Y,Kakizuka A,Takahashi R

MA1-10837 was used in Immunocytochemistry to demonstrate the neuroprotective effects of KUS121, an inhibitor of valosin-containing protein which reduces cellular ATP depletion, in rodent ischemic stroke models.

Thu Aug 08 00:00:00 UTC 2019

KUS121, a valosin-containing protein modulator, attenuates ischemic stroke via preventing ATP depletion.

Scientific reports

Kinoshita H,Maki T,Yasuda K,Kishida N,Sasaoka N,Takagi Y,Kakizuka A,Takahashi R

Thu Aug 08 00:00:00 UTC 2019

Peroxisome proliferator-activated receptor-γ is expressed in eosinophils in nasal polyps.

International archives of allergy and immunology

Asaka C,Honda K,Ito E,Fukui N,Chihara J,Ishikawa K

MA1-10837 was used in immunohistochemistry to investigate the role of peroxisome proliferator-activated receptor gamma in the pathogenesis of nasal polyposis

Wed Jul 13 00:00:00 UTC 2011

Tumor-specific diagnostic marker for transmissible facial tumors of Tasmanian devils: immunohistochemistry studies.

Veterinary pathology

Tovar C,Obendorf D,Murchison EP,Papenfuss AT,Kreiss A,Woods GM

MA1-10837 was used in immunohistochemistry to investigate the expression of proteins in Tasmanian devils with facial tumor disease

Tue Nov 01 00:00:00 UTC 2011

生物信息学

蛋白别名: golli mbp; Golli-mbp; Golli-Mbp; myelin basic protein; Golli-Mbp; myelin basic protein; myelin basic protein S; MBP; MBP S; MGC99675; Myelin A1 protein; Myelin basic protein; myelin basic protein Golli-mbp; myelin basic protein S; myelin deficient; Myelin membrane encephalitogenic protein; shiverer

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基因别名: C76307; golli-mbp; Hmbpr; MBP; Mbps; mld; R75289; Shi

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UniProt ID: (Human) P02686, (Rat) P02688, (Mouse) P04370