Invitrogen

CD4 Monoclonal Antibody (RM4-5), Pacific Blue™

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CD4 Antibody (MCD0428) in Flow

Flow Cytometry analysis using a CD4 monoclonal antibody (Product # MCD0428).

Product Image Gallery

CD4 Antibody — Figure 6 Assessment of epitope frequency in vivo . Splenocytes were harvested from mice that were either immunized with a peptide mixture only (a), sham immunized and challenged with Francisella tularensis LVS (b), or immunized with a peptide mixture and challenged with F. tularensis LVS. (c) Splenocytes were stimulated briefly with lysate from F. tularensis LVS (FT Ag) as a positive control and various peptides; DnaK 109-122, IglB 475-489, and GroEL 466-480. OVA 323-339 peptide was included as a negative control. Brefeldin was introduced for the last 4 hr of culture. Splenocytes were then stained with fluorescent antibodies, as described in the Materials and methods, and analysed for CD4 + IFN-gamma + cells using FACS. Symbols represent stimulation data from individual mice within the different immunization groups and are graphed as CD4 + IFN-gamma + double-positive cells per 10 6 splenocytes. Asterisk, P < 0*05; ns , not significant. {TM}

产品信息

MCD0428

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

RM4-5

抗原

Mouse CD4

偶联物

Pacific Blue™ Pacific Blue™ Pacific Blue™

激发/发射光谱

404/455 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

purified

保存液

PBS with 4mg/mL BSA

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_10372505

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Nanomaterials-Mediated Co-Stimulation of Toll-Like Receptors and CD40 for Antitumor Immunity.

Advanced materials (Deerfield Beach, Fla.)

Yan J,Zhang Y,Du S,Hou X,Li W,Zeng C,Zhang C,Cheng J,Deng B,McComb DW,Zhao W,Xue Y,Kang DD,Cheng X,Dong Y

MCD0428 was used in flow cytometry to develop an immunotherapy regimen that enables co-stimulation of TLR7/8- and CD40-mediated pathways.

Tue Nov 01 00:00:00 UTC 2022

CD8⁺ T cells induce cachexia during chronic viral infection.

Nature immunology

Baazim H,Schweiger M,Moschinger M,Xu H,Scherer T,Popa A,Gallage S,Ali A,Khamina K,Kosack L,Vilagos B,Smyth M,Lercher A,Friske J,Merkler D,Aderem A,Helbich TH,Heikenwälder M,Lang PA,Zechner R,Bergthaler A

MCD0428 was used in Flow Cytometry to identify the role for CD8+ T cells in infection-associated cachexia.

Sat Jun 01 00:00:00 UTC 2019

Mice with an absent stress response are protected against ischemic renal injury.

Kidney international

Sreedharan R,Chen S,Miller M,Haribhai D,Williams CB,Van Why SK

MCD0428 was used in flow cytometry to determine if HSPs ameliorate ischemic renal injury in vivo using HSF-1 KO mice

Mon Sep 01 00:00:00 UTC 2014

Protective role for Toll-like receptor-9 in the development of atherosclerosis in apolipoprotein E-deficient mice.

Arteriosclerosis, thrombosis, and vascular biology

Koulis C,Chen YC,Hausding C,Ahrens I,Kyaw TS,Tay C,Allen T,Jandeleit-Dahm K,Sweet MJ,Akira S,Bobik A,Peter K,Agrotis A

MCD0428 was used in Flow cytometry/Cell sorting to investigate whether TLR9 has a role in the development of atherosclerosis in apolipoprotein E-deficient mice.

Sat Mar 01 00:00:00 UTC 2014

The expression of Sox17 identifies and regulates haemogenic endothelium.

Nature cell biology

Clarke RL,Yzaguirre AD,Yashiro-Ohtani Y,Bondue A,Blanpain C,Pear WS,Speck NA,Keller G

MCD0428 was used in flow cytometry to identify Sox7 as a key regulator of haemogenic endothelial development.

Wed May 01 00:00:00 UTC 2013

Chronic follicular bronchiolitis requires antigen-specific regulatory T cell control to prevent fatal disease progression.

Journal of immunology (Baltimore, Md. : 1950)

Schmitt EG,Haribhai D,Jeschke JC,Co DO,Ziegelbauer J,Yan K,Iwakura Y,Mishra MK,Simpson P,Salzman NH,Williams CB

MCD0428 was used in flow cytometry to examine Treg cell control of chronic autoimmunity in a lymphoreplete host.

Sun Dec 01 00:00:00 UTC 2013

Depletion of B2 but not B1a B cells in BAFF receptor-deficient ApoE mice attenuates atherosclerosis by potently ameliorating arterial inflammation.

PloS one

Kyaw T,Tay C,Hosseini H,Kanellakis P,Gadowski T,MacKay F,Tipping P,Bobik A,Toh BH

MCD0428 was used in flow cytometry to study the development of atherosclerosis in BAFF-R deficient ApoE(-/-) mice.

Mon May 14 00:00:00 UTC 2012

Identification of T-cell epitopes in Francisella tularensis using an ordered protein array of serological targets.

Immunology

Valentino MD,Maben ZJ,Hensley LL,Woolard MD,Kawula TH,Frelinger JA,Frelinger JG

MCD0428 was used in flow cytometry to identify T-cell epitopes of Francisella tularensis.

Tue Mar 01 00:00:00 UTC 2011

Granzyme B regulates antiviral CD8+ T cell responses.

Journal of immunology (Baltimore, Md. : 1950)

Salti SM,Hammelev EM,Grewal JL,Reddy ST,Zemple SJ,Grossman WJ,Grayson MH,Verbsky JW

MCD0428 was used in flow cytometry to determine the role of granzyme B in immune regulation during viral infections.

Thu Dec 15 00:00:00 UTC 2011

Notch3 is dispensable for thymocyte β-selection and Notch1-induced T cell leukemogenesis.

PloS one

Suliman S,Tan J,Xu K,Kousis PC,Kowalski PE,Chang G,Egan SE,Guidos C

MCD0428 was used in flow cytometry to determine the importance of ligand-activated N3 in promoting beta-selection and N1 intracellular domain-induced T cell leukemogenesis

Tue Feb 28 00:00:00 UTC 2012

Therapeutic memory T cells require costimulation for effective clearance of a persistent viral infection.

Journal of virology

Garidou L,Heydari S,Truong P,Brooks DG,McGavern DB

MCD0428 was used in flow cytometry to evaluate immunotherapeutic regimens in mice chronically infected with lymphocytic choriomeningitis virus

Tue Sep 01 00:00:00 UTC 2009

生物信息学

蛋白别名: CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

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基因别名: Cd4; L3T4; Ly-4

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UniProt ID: (Mouse) P06332

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Entrez Gene ID: (Mouse) 12504

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功能

glycoprotein binding protein binding zinc ion binding coreceptor activity enzyme binding protein kinase binding protein homodimerization activity

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