Invitrogen

MCM2 Monoclonal Antibody (2B3)

MCM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MCM2 Antibody (MA5-15895) in IHC (P)

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 monoclonal antibody (Product # MA5-15895) followed with DAB staining.

MCM2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

MCM2 Antibody — Figure 7 PHA-767491 modulates TCR signaling. (A) PHA-767491 treatment suppresses Erk phosphorylation. Immunoblots of OT-I CTL that were not-treated (-) or were treated (+) with PHA-767491 were stimulated with K b -OVA tetramers for the indicated times. Normalized values of individual bands are indicated below the respective bands. Representative blots of at least three independent experiments are shown. (B) The ratio of the band intensities of the phosphorylated proteins to the respective total proteins from (A) are shown in the bar charts, depicted as mean +- SEM. Values represent fold change, normalized to the DMSO-treated sample values for the respective time points. (C) PHA-767491 does not impair Ca 2+ flux. Peripheral lymphocytes were either pre-treated with PHA-767491 or not and were incubated with biotinylated anti-CD3 antibodies. Lymphocytes were first cross-linked using streptavidin, followed by an exogenous addition of CaCl 2 solution, and finally treated with ionomycin. (D) PHA-767491 affects TCR recycling in stimulation assays. Peripheral lymphocytes and thymocytes were stimulated with plate-bound anti-CD3 antibody or anti-CD3/CD28 beads, respectively, for 3 or 17 h. Values indicated in the histograms represent the MFI. (E) Cdc7 association with Dbf4 and MCM2 is reduced in TCR stimulated T cells treated with PHA-767491. Serum-starved Jurkat cells that were not-treated (-) or treated (+) with PHA-767491 were seeded into wells coated with plate-bound anti-CD3 antibo {TM}

产品信息

MA5-15895

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:200-1:1,000

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2B3

抗原

Purified recombinant fragment of human MCM2 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11155194

产品详细信息

MA5-15895 targets MCM2 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.

The MA5-15895 immunogen is purified recombinant fragment of human MCM2 expressed in E. Coli. .

MA5-15895 detects MCM2 which has a predicted molecular weight of approximately 125kDa.

靶标信息

MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. MCM2 has been mapped to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Vitamin B6 deficiency disrupts serotonin signaling in pancreatic islets and induces gestational diabetes in mice.

Communications biology

Fields AM,Welle K,Ho ES,Mesaros C,Susiarjo M

MA5-15895 was used in Immunohistochemistry (Paraffin), Immunohistochemistry to suggest that vitamin B6 deficiency-induced gestational glucose intolerance involves additional mechanisms that are complex and insulin independent.

Fri Mar 26 00:00:00 UTC 2021

Vitamin B6 deficiency disrupts serotonin signaling in pancreatic islets and induces gestational diabetes in mice.

Communications biology

Fields AM,Welle K,Ho ES,Mesaros C,Susiarjo M

Fri Mar 26 00:00:00 UTC 2021

Vitamin B6 deficiency disrupts serotonin signaling in pancreatic islets and induces gestational diabetes in mice.

Communications biology

Fields AM,Welle K,Ho ES,Mesaros C,Susiarjo M

Fri Mar 26 00:00:00 UTC 2021

Co-Administration of Fendiline Hydrochloride Enhances Chemotherapeutic Efficacy of Cisplatin in Neuroblastoma Treatment.

Molecules (Basel, Switzerland)

Brizzolara A,Garbati P,Vella S,Calderoni M,Quattrone A,Tonini GP,Capasso M,Longo L,Barbieri R,Florio T,Pagano A

MA5-15895 was used in Immunohistochemistry to suggest that fendiline hydrochloride can enhance cisplatin anti-tumour activity during neuroblastoma treatment.

Tue Nov 10 00:00:00 UTC 2020

A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation.

Frontiers in immunology

Chen EW,Tay NQ,Brzostek J,Gascoigne NRJ,Rybakin V

Published figure using MCM2 monoclonal antibody (Product # MA5-15895) in Western Blot

Tue Oct 13 00:00:00 UTC 2020

生物信息学

蛋白别名: cell devision cycle-like 1; cyclin-like 1; DNA replication licensing factor MCM2; MGC10606; mini chromosome maintenance deficient 2; minichromosome maintenance deficient 2 (mitotin); minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28

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基因别名: AA959861; AW476101; BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70; KIAA0030; MCM2; Mcmd2; MITOTIN; mKIAA0030

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UniProt ID: (Human) P49736, (Mouse) P97310

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Entrez Gene ID: (Human) 4171, (Rat) 312538, (Mouse) 17216

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