Invitrogen

MCM5 Polyclonal Antibody

MCM5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MCM5 Antibody (PA5-81898) in IHC (P)

Immunohistochemical analysis of MCM5 in human pancreas using MCM5 Polyclonal Antibody (Product # PA5-81898) shows low expression as expected.

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MCM5 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of MCM5 demonstrates antibody specificity. Immunohistochemical analysis of MCM5 using anti-MCM5 Polyclonal Antibody (Product #PA5-81898), shows significant staining of MCM5 in bone marrow and shows minimal or weak staining in pancreas tissues. The relative expression levels of MCM5 within each tissue is shown using RNA-Seq. {RE}

产品信息

PA5-81898

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

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免疫组化（石蜡） (IHC (P))

1:500-1:1,000

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human MCM5. Recombinant protein control fragment (Product #RP-91368).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2789059

产品详细信息

Immunogen sequence: NRYIIMRSGA RQHERDSDRR SSIPITVRQL EAIVRIAEAL SKMKLQPFAT EADVEEALRL FQVSTLDAAL SGTLSGVEGF TSQEDQEMLS RIEKQLKRRF AIGSQVSEHS IIKDFTKQKY PEHAIHKVLQ LMLRRGEIQH RMQRKVLY

靶标信息

The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Towards unravelling biological mechanisms behind radiation-induced oral mucositis via mass spectrometry-based proteomics.

Frontiers in oncology

Subedi P,Huber K,Sterr C,Dietz A,Strasser L,Kaestle F,Hauck SM,Duchrow L,Aldrian C,Monroy Ordonez EB,Luka B,Thomsen AR,Henke M,Gomolka M,Rößler U,Azimzadeh O,Moertl S,Hornhardt S

PA5-81898 was used in Western Blotting to investigate biological mechanisms in keratinocytes post-in vitro ionizing radiation.

Mon Jul 03 00:00:00 UTC 2023

生物信息学

蛋白别名: CDC46 homolog; DNA replication licensing factor MCM5; MCM5 minichromosome maintenance deficient 5, cell division cycle 46; MGC5315; minichromosome maintenance deficient 5 (cell division cycle 46); P1-CDC46

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基因别名: CDC46; MCM5; P1-CDC46

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UniProt ID: (Human) P33992

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Entrez Gene ID: (Human) 4174

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功能

DNA helicase activity chromatin binding DNA replication origin binding protein binding ATP binding DNA metabolism protein

参与通路

G1/S transition of mitotic cell cycle DNA replication DNA replication initiation DNA duplex unwinding