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MCM6 Antibody (PA5-35922) in ICC/IF
Immunofluorescence analysis of MCM6 was performed using 70% confluent PC-3 log phase cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MCM6 Polyclonal Antibody (Product # PA5-35922) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034)... View More
产品信息
PA5-35922
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 682 and 821 of MCM6
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2553232
产品详细信息
Recommended positive controls: 293T, A431, HeLa, HepG2.
Predicted reactivity: Mouse (95%), Rat (96%), Rhesus Monkey (100%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
MCM6 may be involved in the control of a single round of DNA replication during S phase. It binds to chromatin during G1 and detaches from it during S phase as if it licenses the chromatin to replicate.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA replication licensing factor MCM6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); mini chromosome maintenance deficient 6; minichromosome maintenance deficient (mis5, S. pombe) 6; Mis5 homolog; p105MCM
基因别名: ASP-l1; D1Wsu22e; MCG40308; MCM6; Mcmd6; Mis5; P105MCM
UniProt ID: (Human) Q14566, (Mouse) P97311
Entrez Gene ID: (Human) 4175, (Mouse) 17219
