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MCM6 Antibody (PA5-79649) in ICC/IF
Immunocytochemistry analysis of MCM6 using anti-MCM6 antibody (Product # PA5-79649) . MCM6 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-MCM6 antibody (Product # PA5-79649) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
Product Details
PA5-79649
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human MCM6 (589-605aa ESEDFIVEQYKHLRQRD).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746764
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: U87 Cell, COLO320 Cell, HELA Cell, MCF-7 Cell, JURKAT Cell, mouse small intestine tissue, mouse spleen tissue. IHC: Human Intestinal Cancer Tissue, Rat Brain Tissue IHC-F: Human Placenta Tissue, rat intestine tissue. ICC/IF: A431 cell, HELA Cell. Flow: SiHa cell.
Target Information
MCM6 may be involved in the control of a single round of DNA replication during S phase. It binds to chromatin during G1 and detaches from it during S phase as if it licenses the chromatin to replicate.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DNA replication licensing factor MCM6; intestinal DNA replication protein; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); mini chromosome maintenance deficient 6; minichromosome maintenance deficient (mis5, S. pombe) 6; minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); Mis5 homolog; p105MCM
Gene Aliases: ASP-l1; D1Wsu22e; MCG40308; MCM6; Mcmd6; Mis5; P105MCM
UniProt ID: (Human) Q14566, (Mouse) P97311
Entrez Gene ID: (Human) 4175, (Rat) 29685, (Mouse) 17219
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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