Invitrogen

MCP-1 Polyclonal Antibody, PeproTech®

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MCP-1 Antibody (500-P76-1MG) in WB

Western Blot: To detect Rat MCP-1(MCAF) by Western Blot analysis MCP-1 Polyclonal Antibody (Product # 500-P76-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P76-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

免疫组化 (IHC)

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

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中和实验 (Neu)

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其他PubMed (Misc)

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免疫染色 (IS)

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Rat

已发表种属

Pig, Rat

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Rat MCP-1 (CCL2)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930750

产品详细信息

AA Sequence of recombinant protein: QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat MCP-1 (CCL2). Anti-Rat MCP-1 (CCL2)-specific antibody was purified by affinity chromatography employing an immobilized Rat MCP-1 (CCL2) matrix.

Sandwich ELISA: To detect Rat MCP-1 (CCL2) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat MCP-1 (CCL2) (500-P76Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat MCP-1 (CCL2).

Western Blot: To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Activities of Ca²⁺-related ion channels during the formation of kidney stones in an infection-induced urolithiasis rat model.

American journal of physiology. Renal physiology

Cherng JH,Hsu YJ,Liu CC,Tang SH,Sartika D,Chang SJ,Fan GY,Wu ST,Meng E

500-P76 was used in Immunohistochemistry to study the correlation between infection-promoted CaOx kidney stones and alterations in Ca2-related ion channels using a rat model.

Fri Nov 01 00:00:00 UTC 2019

Epothilone B impairs functional recovery after spinal cord injury by increasing secretion of macrophage colony-stimulating factor.

Cell death & disease

Mao L,Gao W,Chen S,Song Y,Song C,Zhou Z,Zhao H,Zhou K,Wang W,Zhu K,Liu C,Mei X

Thu Nov 02 00:00:00 UTC 2017

Epicardial adipose excision slows the progression of porcine coronary atherosclerosis.

Journal of cardiothoracic surgery

McKenney ML,Schultz KA,Boyd JH,Byrd JP,Alloosh M,Teague SD,Arce-Esquivel AA,Fain JN,Laughlin MH,Sacks HS,Sturek M

500-P76 was used in Immunohistochemistry to evaluate the excision of epicardial adipose tissue and its effects on coronary atherosclerosis in pigs.

Fri Jan 03 00:00:00 UTC 2014

Systemic immunotherapy delays photoreceptor cell loss and prevents vascular pathology in Royal College of Surgeons rats.

Molecular vision

Adamus G,Wang S,Kyger M,Worley A,Lu B,Burrows GG

Tue Jan 29 00:00:00 UTC 2013

Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.

Journal of immunology (Baltimore, Md. : 1950)

Rius C,Abu-Taha M,Hermenegildo C,Piqueras L,Cerda-Nicolas JM,Issekutz AC,Estañ L,Cortijo J,Morcillo EJ,Orallo F,Sanz MJ

Wed Sep 15 00:00:00 UTC 2010

Type V collagen-induced oral tolerance plus low-dose cyclosporine prevents rejection of MHC class I and II incompatible lung allografts.

Journal of immunology (Baltimore, Md. : 1950)

Yamada Y,Sekine Y,Yoshida S,Yasufuku K,Petrache I,Benson HL,Brand DD,Yoshino I,Wilkes DS

Wed Jul 01 00:00:00 UTC 2009

Menopause and ovariectomy cause a low grade of systemic inflammation that may be prevented by chronic treatment with low doses of estrogen or losartan.

Journal of immunology (Baltimore, Md. : 1950)

Abu-Taha M,Rius C,Hermenegildo C,Noguera I,Cerda-Nicolas JM,Issekutz AC,Jose PJ,Cortijo J,Morcillo EJ,Sanz MJ

Wed Jul 15 00:00:00 UTC 2009

Force-dependent development of neuropathic central pain and time-related CCL2/CCR2 expression after graded spinal cord contusion injuries of the rat.

Journal of neurotrauma

Knerlich-Lukoschus F,Juraschek M,Blömer U,Lucius R,Mehdorn HM,Held-Feindt J

Thu May 01 00:00:00 UTC 2008

A critical role for TNFalpha in the selective attachment of mononuclear leukocytes to angiotensin-II-stimulated arterioles.

Blood

Mateo T,Naim Abu Nabah Y,Losada M,Estellés R,Company C,Bedrina B,Cerdá-Nicolás JM,Poole S,Jose PJ,Cortijo J,Morcillo EJ,Sanz MJ

Sat Sep 15 00:00:00 UTC 2007

Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines.

Journal of immunology (Baltimore, Md. : 1950)

Mateo T,Abu Nabah YN,Abu Taha M,Mata M,Cerdá-Nicolás M,Proudfoot AE,Stahl RA,Issekutz AC,Cortijo J,Morcillo EJ,Jose PJ,Sanz MJ

Mon May 01 00:00:00 UTC 2006

Antisense to transforming growth factor-beta1 messenger RNA reduces vein graft intimal hyperplasia and monocyte chemotactic protein 1.

Journal of vascular surgery

Wolff RA,Ryomoto M,Stark VE,Malinowski R,Tomas JJ,Stinauer MA,Hullett DA,Hoch JR

500-P76 was used in Enzyme-linked immunosorbent assay to demonstrate that perioperative antisense TGF-beta1 treatment of the vein to be used in arterial reconstructions resulted in a prolonged diminution of intimal hyperplasia (IH).

Tue Mar 01 00:00:00 UTC 2005

Antisense to transforming growth factor-beta1 messenger RNA reduces vein graft intimal hyperplasia and monocyte chemotactic protein 1.

Journal of vascular surgery

Wolff RA,Ryomoto M,Stark VE,Malinowski R,Tomas JJ,Stinauer MA,Hullett DA,Hoch JR

Tue Mar 01 00:00:00 UTC 2005

Expression of monocyte chemoattractant protein-1 in proximal tubular epithelial cells in a rat model of progressive kidney failure.

The Journal of laboratory and clinical medicine

Ota T,Tamura M,Osajima A,Doi Y,Kudo H,Anai H,Miyazaki M,Nishino T,Nakashima Y

Mon Jul 01 00:00:00 UTC 2002

Angiotensin II induces monocyte chemoattractant protein-1 gene expression in rat vascular smooth muscle cells.

Circulation research

Chen XL,Tummala PE,Olbrych MT,Alexander RW,Medford RM

Mon Nov 02 00:00:00 UTC 1998

生物信息学

蛋白别名: C-C motif chemokine 2; H-MCP-1; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; RP23-350G1.3; small inducible cytokine A2; small inducible gene JE; Small-inducible cytokine A2

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基因别名: Ccl2; Je; MCP-1; Mcp1; Scya2; Sigje

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UniProt ID: (Rat) P14844

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Entrez Gene ID: (Rat) 24770

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功能

G-protein coupled receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding cytokine intercellular signal molecule

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