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MCP-3 Antibody (500-M73-500UG) in WB
Western Blot: To detect Human MCP-3 (CCL7) by Western Blot analysis MCP-3 Monoclonal Antibody (Product # 500-M73-500UG) can be used at a concentration of 0.5-1.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-3 (CCL7) is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
产品信息
500-M73-500UG
产品规格
种属反应
Human
宿主/亚型
Mouse
分类
Monoclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human MCP-3 (CCL7)
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929126
产品详细信息
AA Sequence of recombinant protein: QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL.
Preparation: Produced in C3H/HeJ mice immunized with highly pure Recombinant Human MCP-3 (CCL7). Anti-Human MCP-3 (CCL7)-specific antibody was purified from ascites fluid by Protein A affinity chromatography.
Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect Recombinant Human MCP-3 (CCL7) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-3 (CCL7) (500-P37BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.
Western Blot: To detect Human MCP-3 (CCL7) by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-3 (CCL7) is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
靶标信息
This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-C motif chemokine 7; chemokine (C-C motif) ligand 7; H-MCP-3; MCP-3; MGC138463; MGC138465; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; NC28; RP23-350G1.4; small inducible cytokine A7 (monocyte chemotactic protein 3); Small-inducible cytokine A7
基因别名: CCL7; FIC; MARC; MCP-3; MCP3; NC28; SCYA6; SCYA7
UniProt ID: (Human) P80098
Entrez Gene ID: (Human) 6354
monocyte chemotaxis
cellular calcium ion homeostasis
chemotaxis
inflammatory response
cytoskeleton organization
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
regulation of cell shape
response to gamma radiation
positive regulation of cell migration
neutrophil chemotaxis
positive regulation of GTPase activity
eosinophil chemotaxis
positive regulation of inflammatory response
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to ethanol
positive regulation of natural killer cell chemotaxis
