Invitrogen

MCT1 Polyclonal Antibody

MCT1 Antibody in Immunocytochemistry (ICC/IF)

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MCT1 Antibody (PA5-76687) in ICC/IF

Immunofluorescence analysis of MCT1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MCT1 Polyclonal Antibody (Product # PA5-76687) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32790... View More

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MCT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with MCT1 siRNA and reduction of signal was observed in Western Blot using MCT1 Polyclonal Antibody (Product # PA5-76687). {KD}

产品信息

PA5-76687

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

- -

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant full length Human MCT1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2720414

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

SLC16A1 is a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes. Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: FLJ36745; MCT 1; MGC44475; monocarboxylate transporter; Monocarboxylate transporter 1; SLC16A1 protein; solute carrier 16 (monocarboxylic acid transporter), member 1; solute carrier family 16 (monocarboxylate transporter), member 1; solute carrier family 16 (monocarboxylic acid transporters), member 1; Solute carrier family 16 member 1; solute carrier family 16, member 1 (monocarboxylic acid transporter 1)

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基因别名: AL022710; HHF7; MCT; MCT1; MCT1D; RATMCT1; RNMCT1; SLC16A1

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UniProt ID: (Human) P53985, (Rat) P53987, (Mouse) P53986

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Entrez Gene ID: (Human) 6566, (Rat) 25027, (Mouse) 20501

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功能

monocarboxylic acid transmembrane transporter activity lactate transmembrane transporter activity mevalonate transmembrane transporter activity symporter activity protein homodimerization activity organic cyclic compound binding protein binding secondary active monocarboxylate transmembrane transporter activity transporter

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