Abnova

MDFI Monoclonal Antibody (3B4)

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MDFI Antibody (H00004188-M07) in WB

Western Blot analysis of MDFI expression in transfected 293T cell line by MDFI monoclonal antibody (M07), clone 3B4. Lane 1: MDFI transfected lysate(25.029 KDa). Lane 2: Non-transfected lysate. .

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产品信息

H00004188-M07

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

免疫细胞化学 (ICC/IF)

10-100 µg/mL

酶联免疫吸附实验 (ELISA)

10 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

3B4

抗原

MDFI (NP_005577, 33 a.a. approximately 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.45 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Product may be used with Western Blot (Transfected lysate).

Immunogen sequence: PGLEVVTGST HPAEAAPEEG SLEEAATPMP QGNGPGIPQG LDSTDLDVPT EAVTCQPQGN PLGCTPLLPN DSGHPSEL

靶标信息

MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. MDFI an also interfere with the DNA-binding activity of Myod family members and plays an important role in trophoblast and chondrogenic differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。