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ME1 Antibody (MA5-49254) in IHC (P)
Immunohistochemistry analysis of ME1 in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with ME1 Monoclonal antibody (Product # MA5-49254) using a dilution of 2 μg/mL overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG w... View More
Product Details
MA5-49254
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5E5F7
Immunogen
E.coli-derived human ME1 recombinant protein (Position: M1-Q572).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074912
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human Hela whole cell, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue. IHC: human liver cancer tissue, human spleen tissue, rat testis tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytosolic malic enzyme 1; malate dehydrogenase; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); Malic enzyme 1; malic enzyme 1, NADP(+)-dependent, cytosolic; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; malic enzyme, supernatant; NADP-dependent malic enzyme; NADP-ME; pyruvic-malic carboxylase
Gene Aliases: D9Ertd267e; HUMNDME; Mdh-1; ME1; MES; Mod-1; MOD1
UniProt ID: (Human) P48163, (Mouse) P06801
Entrez Gene ID: (Human) 4199, (Mouse) 17436, (Rat) 24552
malic enzyme activity
malate dehydrogenase (decarboxylating) (NAD+) activity
malate dehydrogenase (decarboxylating) (NADP+) activity
oxaloacetate decarboxylase activity
electron carrier activity
manganese ion binding
ADP binding
NADP binding
NAD binding
protein binding
oxidoreductase activity
metal ion binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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