Invitrogen

MECP2 Polyclonal Antibody

MECP2 Antibody in Immunocytochemistry (ICC/IF)

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MECP2 Antibody (PA1-887) in ICC/IF

Immunofluorescence analysis of Mecp2 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MECP2 Polyclonal Antibody (Product # PA1-887) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:2000), f... View More

MECP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MECP2 Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-887

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1500

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:2,000

免疫细胞化学 (ICC/IF)

1:100-1:200

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Fruit fly, Human, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: M(1) V A G M L G L R E E K S E D(15) C

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2143845

产品详细信息

PA1-887 detects methyl CpG binding protein 2 (MeCP2) from human, mouse and rat tissues and cells.

PA1-887 has been successfully used in Western blot, IHC-P, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~56 kDa protein representing MeCP2 from AtT20 cell extract.

PA1-887 immunizing peptide corresponds to amino acid residues 1-15 from mouse MeCP2. This sequence is completely conserved in human MeCP2. PA1-887 immunizing peptide (Cat. # PEP-120) is available for use in neutralization and control experiments.

靶标信息

MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3 and MBD4) that have a methyl-CpG binding domain. MECP2 is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. MECP2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

MECP2 impairs neuronal structure by regulating KIBRA.

Neurobiology of disease

Williams AA,White R,Siniard A,Corneveaux J,Huentelman M,Duch C

PA1-887 was used in Western Blotting to identify memory associated KIBRA as a target of MECP2 in regulating dendritic growth.

Fri Jul 01 00:00:00 UTC 2016

Apoptotic Activity of MeCP2 Is Enhanced by C-Terminal Truncating Mutations.

PloS one

Williams AA,Mehler VJ,Mueller C,Vonhoff F,White R,Duch C

PA1-887 was used in western blot study the function of the MeCP2 C-terminus

Mon Jul 31 00:00:00 UTC 2017

Rett Syndrome Mutant Neural Cells Lacks MeCP2 Immunoreactive Bands.

PloS one

Bueno C,Tabares-Seisdedos R,Moraleda JM,Martinez S

PA1-887 was used in Western Blotting to advance understanding of multiple MeCP2 immunoreactive bands in control neural cells and p.T158M MeCP2e1 mutant cells.

Thu Aug 25 00:00:00 UTC 2016

The Effects of Maternal Separation on Adult Methamphetamine Self-Administration, Extinction, Reinstatement, and MeCP2 Immunoreactivity in the Nucleus Accumbens.

Frontiers in psychiatry

Lewis CR,Staudinger K,Scheck L,Olive MF

PA1-887 was used in immunohistochemistry to study adult methamphetamine self-administration, methamphetamine-seeking behaviour and nucleus accumbens MeCP2 levels following maternal separation in rats