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MED10 Antibody (PA5-100608) in ICC/IF
Immunocytochemistry analysis of MED10 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with MED10 polyclonal antibody (Product # PA5-100608) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-100608
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MED10(Accession Q9BTT4), corresponding to amino acid residues E10-D60.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2850115
产品详细信息
Antibody detects endogenous levels of total MED10.
靶标信息
MED10 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Mediator complex subunit 10; Mediator of RNA polymerase II transcription subunit 10; mediator of RNA polymerase II transcription, subunit 10; mediator of RNA polymerase II transcription, subunit 10 homolog (NUT2, S. cerevisiae); Transformation-related gene 17 protein; Transformation-related gene 20 protein; TRG-17; TRG-20
基因别名: AA959813; AI385605; C78613; D13Wsu50e; L6; MED10; NUT2; RGD1310128; TRG17; TRG20
UniProt ID: (Human) Q9BTT4, (Mouse) Q9CXU0
Entrez Gene ID: (Human) 84246, (Rat) 290939, (Mouse) 28077
transcription initiation from RNA polymerase II promoter
protein ubiquitination
stem cell population maintenance
positive regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
