Invitrogen

MED17 Polyclonal Antibody

2 Published Figures

View all (9) MED17 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (4)
Published Figures (2)

MED17 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MED17 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MED17 Antibody (PA5-40839) in IHC (P)

Immunohistochemistry (paraffin-embedded) analysis of human neural tissue using an anti-CRSP6 polyclonal antibody (Product # PA5-40839).

MED17 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MED17 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MED17 Antibody in Western Blot (WB)

MED17 Antibody in ChIP Assay (CHIP)

MED17 Antibody in Western Blot, Immunoprecipitation, ChIP Assay (WB, IP, ChIP)

MED17 Antibody in Western Blot, Immunoprecipitation, ChIP Assay (WB, IP, ChIP)

Product Image Gallery

MED17 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (paraffin-embedded) analysis of human neural tissue using an anti-CRSP6 polyclonal antibody (Product # PA5-40839).

MED17 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (paraffin-embedded) analysis of human brain cells using an anti-CRSP6 polyclonal antibody (Product # PA5-40839).

MED17 Antibody in Western Blot (WB) — Western blot analysis of human HepG2 cell lysate using an anti-CRSP6 polyclonal antibody (Product # PA5-40839).

MED17 Antibody in ChIP Assay (CHIP) — ChIP assay analysis of quiescent human colon carcinoma HCT116 cells using an anti-CRSP6 polyclonal antibody (Product # PA5-40839).

MED17 Antibody in Western Blot, Immunoprecipitation, ChIP Assay (WB, IP, ChIP) — Fig. 8 PLK3 controls GOF p53's transactivation and binding to its inducible gene promoters. a Transient transactivation assay of H1299 cells transfected with the p53-R273H (or empty vector) along with siRNA against PLK3 (or scrambled control) and the cyclin A promoter, Chk1 promoter, or the TERT promoter-luciferase construct. Transient transcriptional assays were carried out in H1299 cells. Cells were harvested after 24 h of transfection. Firefly luciferase readings were plotted as relative light units (RLU) and is presented as the mean +- SEM ( n = 3, performed twice). A two-sided Student's t -test was performed and CCNA2 * p = 0.01, *** p = 6.5E-7; Chk1 *** p < 2.5E-6; TERT * p = 0.01. b H1975 PLK3 KO cells were used to determine the ability of GOF p53 to upregulate its activated genes. Transcript levels of PLK3, Chk1, CCNA2, and EIF3C were measured by QPCR. Data are normalized with GAPDH and are presented as the mean +- SEM ( n = 3). A two-sided Student's t -test was performed and PLK3 ** p = 0.002; Chk1 ** p = 0.001; CCNA2 * p = 0.03; EIF3C * p = 0.02. c Co-immunoprecipitation of mutant p53 and PLK3 in murine and human cells. Mouse R172H and human R273H were able to interact with PLK3 but their transactivation-deficient counterparts, L25Q/W26S/R172H and L22Q/W23S/R273H were not. d PLK3 knockout (KO) cells were used to determine whether the interaction between mutant p53 and Med17 has any requirement for PLK3. Co-immunoprecipitation in H1975 PLK3 KO cells versus control sh

MED17 Antibody in Western Blot, Immunoprecipitation, ChIP Assay (WB, IP, ChIP) — Fig. 4 GOF p53 interacts with Med17 and amino acid acids at mouse p53 L25, W26 (L22, W23 in human) are important for this interaction. a Immunoprecipitation experiment showing complex formation between murine p53-R172H and the Mediator protein 17 (Med17) in solution. Nuclear extracts were made from murine lung cells expressing p53-R172H (generated by adeno Cre as described in the text), p53-L25Q/W26S/R172H, and control, and immunoprecipitated by Med17 antibody and probed with anti-p53 antibody. b Immunoprecipitation experiment showing complex formation between p53-R273H with Med17 in solution. Nuclear extracts were prepared from H1299 cells stably transfected with empty vector, expressing p53-R273H and -L22Q/W23S/R273H, and immunoprecipitated by Med17 antibody and probed with anti-p53 antibody. c Immunoprecipitation experiment showing complex formation between p53-R175H and Med17 in solution. Nuclear extracts were prepared from H1299 cells stably transfected with empty vector, expressing p53-R175H and -L22Q/W23S/R175H, and immunoprecipitated by Med17 antibody and probed with anti-p53 antibody. d Reverse immunoprecipitation experiment showing complex formation between murine p53-R172H and the Mediator protein 17 (Med17) in solution. Whole cell extracts were made from murine lung cells expressing p53-R172H (generated by adeno Cre as described in the text), p53-L25Q/W26S/R172H, and control after transfection with Flag-Med17, and immunoprecipitated by p53 antibody and probed with

Product Details

PA5-40839

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

4-8 µg/mL

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

ChIP assay (ChIP)

0.5 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the N-terminal of human CRSP6 (aa 156-205).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2576978

Product Specific Information

Peptide sequence: AAQILLKGAE RLTKSVTENQ ENKLQRDFNS ELLRLRQHWK LRKVGDKILG

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 100%

Target Information

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by CRSP6 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e. g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The oncogenicity of tumor-derived mutant p53 is enhanced by the recruitment of PLK3.

Nature communications

Vaughan CA,Singh S,Subler MA,Windle JJ,Inoue K,Fry EA,Pillappa R,Grossman SR,Windle B,Andrew Yeudall W,Deb SP,Deb S

PA5-40839 was used in Immunoprecipitation, ChIP assay to show a mechanistic involvement of PLK3 in mutant p53 pathway of oncogenesis.

Fri Jan 29 00:00:00 UTC 2021

The oncogenicity of tumor-derived mutant p53 is enhanced by the recruitment of PLK3.

Nature communications

Vaughan CA,Singh S,Subler MA,Windle JJ,Inoue K,Fry EA,Pillappa R,Grossman SR,Windle B,Andrew Yeudall W,Deb SP,Deb S

PA5-40839 was used in Immunoprecipitation, ChIP assay to show a mechanistic involvement of PLK3 in mutant p53 pathway of oncogenesis.

Fri Jan 29 00:00:00 UTC 2021

The oncogenicity of tumor-derived mutant p53 is enhanced by the recruitment of PLK3.

Nature communications

Vaughan CA,Singh S,Subler MA,Windle JJ,Inoue K,Fry EA,Pillappa R,Grossman SR,Windle B,Andrew Yeudall W,Deb SP,Deb S

PA5-40839 was used in Immunoprecipitation, ChIP assay to show a mechanistic involvement of PLK3 in mutant p53 pathway of oncogenesis.

Fri Jan 29 00:00:00 UTC 2021

The oncogenicity of tumor-derived mutant p53 is enhanced by the recruitment of PLK3.

Nature communications

Vaughan CA,Singh S,Subler MA,Windle JJ,Inoue K,Fry EA,Pillappa R,Grossman SR,Windle B,Andrew Yeudall W,Deb SP,Deb S

PA5-40839 was used in Immunoprecipitation, ChIP assay to show a mechanistic involvement of PLK3 in mutant p53 pathway of oncogenesis.

Fri Jan 29 00:00:00 UTC 2021

The oncogenicity of tumor-derived mutant p53 is enhanced by the recruitment of PLK3.

Nature communications

Vaughan CA,Singh S,Subler MA,Windle JJ,Inoue K,Fry EA,Pillappa R,Grossman SR,Windle B,Andrew Yeudall W,Deb SP,Deb S

PA5-40839 was used in Immunoprecipitation, ChIP assay to show a mechanistic involvement of PLK3 in mutant p53 pathway of oncogenesis.

Fri Jan 29 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: Activator-recruited cofactor 77 kDa component; ARC77; Cofactor required for Sp1 transcriptional activation subunit 6; cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa; CRSP complex subunit 6; DRIP80; Mediator complex subunit 17; Mediator of RNA polymerase II transcription subunit 17; Thyroid hormone receptor-associated protein complex 80 kDa component; Transcriptional coactivator CRSP77; Trap80; Vitamin D3 receptor-interacting protein complex 80 kDa component

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Gene Aliases: ARC77; CRSP6; CRSP77; DRIP77; DRIP80; MED17; TRAP80

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UniProt ID: (Human) B3KN07

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Entrez Gene ID: (Human) 9440

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RNA polymerase II transcription cofactor activity transcription cofactor activity transcription coactivator activity receptor activity protein binding ligand-dependent nuclear receptor transcription coactivator activity vitamin D receptor binding thyroid hormone receptor binding ubiquitin protein ligase activity RNA metabolism protein nucleic acid metabolism protein general transcription factor

regulation of transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter protein ubiquitination stem cell population maintenance intracellular steroid hormone receptor signaling pathway androgen receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter

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