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MED20 Antibody (PA5-90677) in WB
Western blot analysis of MED20 in extracts of various cell lines. Samples were incubated with MED20 Polyclonal antibody (Product # PA5-90677) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-90677
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human MED20 (NP_0042662)
偶联物
形式
Liquid
浓度
1.32 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2806170
产品详细信息
Immunogen sequence: MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQTG KLMYVMHNSE YPLSCFALFE NGPCLIADTN FDVLMVKLKG FFQSAKASKI ETRGTRYQYC DFLVKVGTVT MGPSARGISV EVEYGPCVVA SDCWSLLLEF LQSFLGSHTP GAPAVFGNRH DAVYGPADTM VQYMELFNKI RKQQQVPVAG IR; Positive Samples: DU145, Mouse brain; Cellular Location: Nucleus
靶标信息
MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II, and general initiation factors.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hTRFP; Mediator complex subunit 20; Mediator of RNA polymerase II transcription subunit 20; Trf (TATA binding protein-related factor)-proximal homolog; Trf (TATA binding protein-related factor)-proximal protein homolog; TRF-proximal protein homolog; ubiquitin specific protease 49
基因别名: 1110011O05Rik; 2410115I17Rik; AU018348; MED20; PRO0213; TRFP; Usp49
UniProt ID: (Human) Q9H944, (Mouse) Q9R0X0, (Rat) Q5XIE9
Entrez Gene ID: (Human) 9477, (Mouse) 56771, (Rat) 316209
