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MED22 Antibody (PA5-89465) in WB
Western blot analysis of MED22 in extracts of various cell lines. Samples were incubated with MED22 Polyclonal antibody (Product # PA5-89465) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
产品信息
PA5-89465
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human MED22 (NP_8524681)
偶联物
形式
Liquid
浓度
0.58 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805588
产品详细信息
Immunogen sequence: MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ RNQQLRTLQE ECDRKLITLR DEISIDLYEL EEEYYSSRYK; Positive Samples: U-87MG, A-431, A-549, Mouse testis; Cellular Location: Nucleus
靶标信息
This gene is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Mediator complex subunit 22; Mediator of RNA polymerase II transcription subunit 22; MGC48682; Surf-5; surfeit 5; surfeit gene 5; Surfeit locus protein 5
基因别名: AW212655; AW558812; MED22; MED24; RGD1564893; Surf-5; SURF5
UniProt ID: (Human) Q15528, (Rat) A0JPN6, (Mouse) Q62276
Entrez Gene ID: (Human) 6837, (Rat) 499762, (Mouse) 20933
