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MED31 Antibody (PA5-90741) in WB
Western blot analysis of MED31 in extracts of various cell lines. Samples were incubated with MED31 Polyclonal antibody (Product # PA5-90741) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
产品信息
PA5-90741
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human MED31 (NP_0571441)
偶联物
形式
Liquid
浓度
2.8 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2806215
产品详细信息
Immunogen sequence: MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN YLKYLLYWKD PEYAKYLKYP QCLHMLELLQ YEHFRKELVN AQCAKFIDEQ QILHWQHYSR KRMRLQQALA EQQQQNNTSG K; Positive Samples: U-87MG, Mouse brain, Mouse liver, Mouse testis, Rat brain; Cellular Location: Nucleus
靶标信息
MED31 is the component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: FLJ27436; FLJ36714; hSOH1; Mediator complex subunit 31; Mediator complex subunit SOH1; Mediator of RNA polymerase II transcription subunit 31; mediator of RNA polymerase II transcription, subunit 31 homolog
基因别名: 3110004H13Rik; CGI-125; l11Jus15; MED31; RGD1309457; SOH1
UniProt ID: (Human) Q9Y3C7, (Mouse) Q9CXU1
Entrez Gene ID: (Human) 51003, (Rat) 287475, (Mouse) 67279
regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
protein ubiquitination
negative regulation of fibroblast proliferation
limb development
transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
