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MEK2 Antibody (PA5-118046) in ICC/IF
Immunofluorescence staining of MEK2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with MEK2 Polyclonal Antibody (Product # PA5-118046, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
产品信息
PA5-118046
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived Human MEK2/MAP2K2 fragment
偶联物
形式
Liquid
纯化类型
Antigen affinity chromatography, Protein A
保存液
PBS, pH 7
内含物
0.03% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2902650
靶标信息
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that is known to play a critical role in mitogen growth factor signal transduction. Mitogen activated protein kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; ERK kinase 2; FLJ26075; MAP kinase kinase 2; MAP2K1; MAPK; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MEK 1; MEK 2; MEK-1; MEK-2; mitogen-activated protein kinase kinase 2, p45; MKK1; OTTHUMP00000165827; PRKMK1
基因别名: CFC4; MAP2K2; MAPKK2; MEK2; MKK2; PRKMK2
UniProt ID: (Human) P36507
Entrez Gene ID: (Human) 5605
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein serine/threonine/tyrosine kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
PDZ domain binding
protein complex scaffold
protein serine/threonine kinase activator activity
metal ion binding
scaffold protein binding
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPK activity
heart development
negative regulation of gene expression
peptidyl-tyrosine phosphorylation
thyroid gland development
regulation of stress-activated MAPK cascade
proteolysis in other organism
peptidyl-serine autophosphorylation
positive regulation of transcription, DNA-templated
thymus development
regulation of axon regeneration
positive regulation of axonogenesis
face development
trachea formation
epithelial cell proliferation involved in lung morphogenesis
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of protein serine/threonine kinase activity
regulation of Golgi inheritance
positive regulation of production of miRNAs involved in gene silencing by miRNA
positive regulation of cell motility
regulation of early endosome to late endosome transport
