Invitrogen

MEK6 Monoclonal Antibody (3H12B9)

MEK6 Antibody in Immunocytochemistry (ICC/IF)

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MEK6 Antibody (MA5-15809) in ICC/IF

Immunofluorescence analysis of MEK6 was performed using 70% confluent log phase HeLa and HCT 116 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MEK6 Monoclonal Antibody (3H12B9) (Product # MA5-15809) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More

MEK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MEK6 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of Anti-MEK6 was observed in Hep G2, HEK-293, HeLa, NIH/3T3, Mouse cerebellum, Mouse white adipose and Mouse colon in comparison to HCT 116, SK-OV-3 and K-562, Mouse skeletal muscle using Anti-MEK6 Monoclonal Antibody (3H12B9) (Product # MA5-15809) in Western Blot. {RE}

产品信息

MA5-15809

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:200

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3H12B9

抗原

Purified recombinant fragment of human MAP2K6 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11155010

产品详细信息

MA5-15809 targets MAP2K6 in indirect ELISA, IHC and WB applications and shows reactivity with Human and mouse samples.

The MA5-15809 immunogen is purified recombinant fragment of human MAP2K6 expressed in E. Coli.

MA5-15809 detects MAP2K6 which has a predicted molecular weight of approximately 38kDa.

靶标信息

MKK6 (MEK6, MAP2K6) is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. Tissue specificity: Isoform 2 is only expressed in skeletal muscle. Isoform 1, on the other hand, is found in skeletal muscle, heart, and in lesser extent in liver or pancreas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Dual specificity mitogen-activated protein kinase kinase 6; MAP kinase kinase 6; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MAPKK3; MEK 3; MEK 6; MEK3; mitogen activated protein kinase kinase 6; MKK3; PRKMK3; protein kinase, mitogen activated, kinase 6; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPK kinase 2; SAPK kinase 3; SAPKK-2; SAPKK2; SAPKK3; Stress-activated protein kinase kinase 3

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基因别名: MAP2K6; MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3; SAPKK3; SKK3

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UniProt ID: (Human) P52564, (Mouse) P70236

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Entrez Gene ID: (Human) 5608, (Mouse) 26399

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