Bethyl Laboratories

MEKK2 Polyclonal Antibody

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MEKK2 Antibody (A302-164A) in WB

Detection of human MEKK2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MEKK2 antibody (Product # A302-164A) used for WB at 0.04 µg/mL (A) and 0.4 µg/mL (B) and used for IP at 3 µg/mg lysate. MEKK2 was also immunoprecipitated by rabbit anti-MEKK2 antibody A302-163A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

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产品信息

A302-164A

应用

建议稀释比

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免疫印迹 (WB)

1:2,000-1:10,000

免疫沉淀 (IP)

2-5 µg/mg lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 125 and 175 of human MAPK/ERK kinase kinase 2 (mitogen-activated protein kinase kinase kinase 2).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.20 mg/mL

纯化类型

Antigen affinity chromatography

保存液

TBS, pH 7.0 to 8.0, with 0.1% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

靶标信息

MAP3K2 (MEKK2) is a serine/threonine kinase that activates kinases involved in the MAP kinase signaling pathway. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process.

仅用于科研。不用于诊断过程。未经明确授权不得转售。