Rockland

MEN1 Polyclonal Antibody

产品信息

200-901-MJ8

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

免疫组化 (IHC)

1:500

酶联免疫吸附实验 (ELISA)

1:10,000-1:50,000

产品规格

种属反应

Human

宿主/亚型

Chicken / IgY

分类

Polyclonal

类型

Antibody

抗原

Anti-Menin1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a C-Terminal portion of human MEN1 conjugated to Keyhole Limpet Hemocyanin (KLH).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

selective precipitation

保存液

PBS, pH 7

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human Menin1. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Blast analysis the immunogen sequence shows 100% identity withhuman, mouse, rat, bovine, canine, and is highly conserved.

靶标信息

Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.

仅用于科研。不用于诊断过程。未经明确授权不得转售。