Invitrogen

MEPCE Polyclonal Antibody

查看其他9个MEPCE抗体

抗体检测数据 (5)

MEPCE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MEPCE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MEPCE Antibody (PA5-100105) in IHC (P)

Immunohistochemistry analysis of MEPCE in human kidney cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MEPCE polyclonal antibody (Product # PA5-100105) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MEPCE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MEPCE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MEPCE Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MEPCE Antibody in Western Blot (WB)

MEPCE Antibody in Western Blot (WB)

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MEPCE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MEPCE in human kidney cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MEPCE polyclonal antibody (Product # PA5-100105) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MEPCE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MEPCE in rat stomach tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MEPCE polyclonal antibody (Product # PA5-100105) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MEPCE Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MEPCE in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with MEPCE polyclonal antibody (Product # PA5-100105) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

MEPCE Antibody in Western Blot (WB) — Western blot analysis of MEPCE in 293 cell lysate (left lane: treated with blocking peptide). Samples were incubated with MEPCE polyclonal antibody (Product # PA5-100105).

MEPCE Antibody in Western Blot (WB) — Western blot analysis of MEPCE in HeLa cells. Samples were incubated with MEPCE polyclonal antibody (Product # PA5-100105).

产品信息

PA5-100105

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

产品规格

种属反应

Human, Mouse, Rat, Zebrafish

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human MEPCE(Accession Q7L2J0), corresponding to amino acid residues A345-H395.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2815635

产品详细信息

Antibody detects endogenous levels of total MEPCE.

靶标信息

S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it (PubMed:17643375, PubMed:19906723, PubMed:30559425). Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (PubMed:17643375). The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC) (PubMed:28254838). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex (PubMed:19906723). MEPCE has a non-enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center (PubMed:19906723). [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 7SK snRNA methylphosphate capping enzyme; bcdin3; Bicoid-interacting protein 3 homolog; Bin3 homolog; bin3, bicoid-interacting 3; bin3, bicoid-interacting 3, homolog; FLJ20257; MePCE

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基因别名: BCDIN3; Bipl1; D5Wsu46e; fc59f06; MEPCE; si:ch211-283h6.1; wu:fc59f06; zBCDIN3

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UniProt ID: (Human) Q7L2J0, (Mouse) Q8K3A9

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Entrez Gene ID: (Human) 56257, (Rat) 304361, (Mouse) 231803, (Zebrafish) 561384

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RNA methyltransferase activity S-adenosylmethionine-dependent methyltransferase activity poly(A) RNA binding rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity snRNA binding RNA 5'-methyltransferase activity

negative regulation of transcription from RNA polymerase II promoter RNA methylation snRNA metabolic process methylation negative regulation of chromatin binding snRNA modification positive regulation of G1/S transition of mitotic cell cycle positive regulation of protein localization to Cajal body positive regulation of snRNA transcription by RNA polymerase II