Invitrogen

METTL7B Polyclonal Antibody

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抗体检测数据 (4)

METTL7B Antibody in Immunocytochemistry (ICC/IF)

METTL7B Antibody in Immunocytochemistry (ICC/IF)

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METTL7B Antibody (PA5-121075) in ICC/IF

Immunofluorescence analysis of METTL7B in U-251MG cells. Samples were incubated with METTL7B Polyclonal antibody (Product # PA5-121075) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

METTL7B Antibody in Immunocytochemistry (ICC/IF)

METTL7B Antibody in Immunocytochemistry (ICC/IF)

METTL7B Antibody in Western Blot (WB)

METTL7B Antibody in Western Blot (WB)

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METTL7B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of METTL7B in U-251MG cells. Samples were incubated with METTL7B Polyclonal antibody (Product # PA5-121075) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

METTL7B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of METTL7B in HepG2 cells. Samples were incubated with METTL7B Polyclonal antibody (Product # PA5-121075) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

METTL7B Antibody in Western Blot (WB) — Western blot analysis of METTL7B in extracts of various cell lines. Samples were incubated with METTL7B Polyclonal antibody (Product # PA5-121075) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

METTL7B Antibody in Western Blot (WB) — Western blot analysis of METTL7B in extracts of Rat liver. Samples were incubated with METTL7B Polyclonal antibody (Product # PA5-121075) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

产品信息

PA5-121075

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 165-244 of human METTL7B (NP_689850.2).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.544 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.05% ProClin 300

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2914647

产品详细信息

Positive test controls include: HT-29, Mouse liver, Rat liver.

Immunogen sequence: RPGGVLFFWE HVAEPYGSWA FMWQQVFEPT WKHIGDGCCL TRETWKDLEN AQFSEIQMER QPPPLKWLPV GPHIMGKAVK

靶标信息

MettL7B belongs to the methyltransferase superfamily. It is a probable methyltransferase. Methyltransferase is a type of transferase enzyme which transfers a methyl group from a donor to an acceptor. Often methylation occurs on nucleic bases in DNA or amino acids in protein structures. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence. DNA methylation may be necessary for normal growth from embryonic stages in mammals. When mutant embryonic stem cells lacking the murine DNA methyltransferase gene were introduced to a germline of mice they caused a recessive lethal phenotype. Methylation may also be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ALDI; Associated with lipid droplet protein 1; associated with lipid droplets 1; Methyltransferase-like protein 7B; Thiol S-methyltransferase METTL7B; Thiol S-methyltransferase TMT1B

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基因别名: 0610006F02Rik; AI266817; ALDI; METTL7B; RGD1305205; TMT1B; UNQ594/PRO1180

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UniProt ID: (Human) Q6UX53, (Rat) Q562C4, (Mouse) Q9DD20

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Entrez Gene ID: (Human) 196410, (Rat) 366792, (Mouse) 71664

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S-adenosylmethionine-dependent methyltransferase activity rRNA (adenine-N6,N6-)-dimethyltransferase activity molecular_function protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity methyltransferase

methylation biological_process metabolic process