Invitrogen

MGMT Monoclonal Antibody (MT 23.2)

MGMT Antibody in Immunocytochemistry (ICC/IF)

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MGMT Antibody (MA3-16537) in ICC/IF

Immunofluorescence analysis of Methylated-DNA-protein-cysteine methyltransferase was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MGMT Monoclonal Antibody (MT 23.2) (Product # MA3-16537) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Second... View More

MGMT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MGMT Antibody in Immunohistochemistry (IHC)

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产品信息

MA3-16537

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

1:500-1:1,000

查看6篇已发表文章 6篇已发表文章

免疫细胞化学 (ICC/IF)

1:500

流式细胞分析 (Flow)

1-2 µg/mL

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

MT 23.2

抗原

Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_568616

产品详细信息

Suggested positive control: CEM-CCRF cells.

TK6 cells as a negative control.

靶标信息

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytostatic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phase 2 study of concurrent radiotherapy and temozolomide followed by temozolomide and lomustine in the treatment of children with high-grade glioma: a report of the Children's Oncology Group ACNS0423 study.

Neuro-oncology

Jakacki RI,Cohen KJ,Buxton A,Krailo MD,Burger PC,Rosenblum MK,Brat DJ,Hamilton RL,Eckel SP,Zhou T,Lavey RS,Pollack IF

MA3-16537 was used in immunohistochemistry - paraffin section to analyze the Children's Oncology Group ACNS0423 phase 2 study of concurrent temozolomide and radiotherapy followed by lomustine and temozolomide in treatment of children with high-grade glio

Sat Oct 01 00:00:00 UTC 2016

Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype.

PloS one

Munthe S,Petterson SA,Dahlrot RH,Poulsen FR,Hansen S,Kristensen BW

MA3-16537 was used in immunohistochemistry - paraffin section to study tumor periphery glioma cells and their stem cell phenotype

Mon Jul 10 00:00:00 UTC 2017

Long-term outcome and MGMT as a predictive marker in 24 patients with atypical pituitary adenomas and pituitary carcinomas given treatment with temozolomide.

The Journal of clinical endocrinology and metabolism

Bengtsson D,Schrøder HD,Andersen M,Maiter D,Berinder K,Feldt Rasmussen U,Rasmussen ÅK,Johannsson G,Hoybye C,van der Lely AJ,Petersson M,Ragnarsson O,Burman P

MA3-16537 was used in immunohistochemistry - paraffin section to discuss the use of temozolomide to treat pituitary tumors

Wed Apr 01 00:00:00 UTC 2015

MGMT expression and pituitary tumours: relationship to tumour biology.

Pituitary

McCormack A,Kaplan W,Gill AJ,Little N,Cook R,Robinson B,Clifton-Bligh R

MA3-16537 was used in immunohistochemistry to perform a comparative study the gene expression profiles and cancer biology of pituitary tumors classified as high or low expressors of MGMT

Sat Jun 01 00:00:00 UTC 2013

Hsp27 (HSPB1): a possible surrogate molecular marker for loss of heterozygosity (LOH) of chromosome 1p in oligodendrogliomas but not in astrocytomas.

Cell stress & chaperones

Castro GN,Cayado-Gutiérrez N,Moncalero VL,Lima P,De Angelis RL,Chávez V,Cuello-Carrión FD,Ciocca DR

MA3-16537 was used in immunohistochemistry (paraffin) to examine heat shock proteins in different subtypes of gliomas.

Thu Nov 01 00:00:00 UTC 2012

O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation is a rare event in soft tissue sarcoma.

Radiation oncology (London, England)

Jakob J,Hille M,Sauer C,Ströbel P,Wenz F,Hohenberger P

MA3-16537 was used in immunohistochemistry to study the frequency of MGMT promoter methylation in soft tissue sarcomas

Tue Oct 30 00:00:00 UTC 2012

O6-Methylguanine-DNA methyltransferase protein expression by immunohistochemistry in brain and non-brain systemic tumours: systematic review and meta-analysis of correlation with methylation-specific polymerase chain reaction.

BMC cancer

Brell M,Ibáñez J,Tortosa A

MA3-16537 was used in flow cytometry and immunohistochemistry to review methods to analyze O6-Methylguanine-DNA methyltransferase

Wed Jan 26 00:00:00 UTC 2011

Impact of morphology, MIB-1, p53 and MGMT on outcome in pilocytic astrocytomas.

Brain pathology (Zurich, Switzerland)

Horbinski C,Hamilton RL,Lovell C,Burnham J,Pollack IF

MA3-16537 was used in immunohistochemistry - paraffin section to discuss indicators of tumor aggressiveness for pilocytic astrocytoma

Sat May 01 00:00:00 UTC 2010

Salvage therapy with temozolomide in patients with aggressive or metastatic pituitary adenomas: experience in six cases.

European journal of endocrinology

Losa M,Mazza E,Terreni MR,McCormack A,Gill AJ,Motta M,Cangi MG,Talarico A,Mortini P,Reni M

MA3-16537 was used in immunohistochemistry to discuss the therapeutic benefic of temozolomide treatment in patients with aggressive or metastatic pituitary adenomas

Wed Dec 01 00:00:00 UTC 2010

Low O6-methylguanine-DNA methyltransferase (MGMT) expression and response to temozolomide in aggressive pituitary tumours.

Clinical endocrinology

McCormack AI,McDonald KL,Gill AJ,Clark SJ,Burt MG,Campbell KA,Braund WJ,Little NS,Cook RJ,Grossman AB,Robinson BG,Clifton-Bligh RJ

MA3-16537 was used in immunohistochemistry to investigate the association between O6-methylguanine-DNA methyltransferase expression and temozolomide therapy in aggressive pituitary tumours

Sat Aug 01 00:00:00 UTC 2009

Low O6-methylguanine-DNA methyltransferase (MGMT) expression and response to temozolomide in aggressive pituitary tumours.

Clinical endocrinology

McCormack AI,McDonald KL,Gill AJ,Clark SJ,Burt MG,Campbell KA,Braund WJ,Little NS,Cook RJ,Grossman AB,Robinson BG,Clifton-Bligh RJ

MA3-16537 was used in immunohistochemistry to investigate the association between O6-methylguanine-DNA methyltransferase expression and temozolomide therapy in aggressive pituitary tumours

Sat Aug 01 00:00:00 UTC 2009

Prognostic significance of DNA repair proteins MLH1, MSH2 and MGMT expression in non-small-cell lung cancer and precursor lesions.

Histopathology

Cooper WA,Kohonen-Corish MR,Chan C,Kwun SY,McCaughan B,Kennedy C,Sutherland RL,Lee CS

MA3-16537 was used in immunohistochemistry - paraffin section to investigate the role of DNA repair proteins and their prognostic significance in non-small-cell lung cancer

Tue Apr 01 00:00:00 UTC 2008

生物信息学

蛋白别名: 6-O-methylguanine-DNA methyltransferase; methylated DNA protein cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; MGMT; O-6-alkylguanine-DNA alkyltransferase; O-6-methylguan; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase

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基因别名: Agat; AGT; AI267024; MGMT

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UniProt ID: (Human) P16455, (Mouse) P26187

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Entrez Gene ID: (Human) 4255, (Mouse) 17314

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功能

DNA binding damaged DNA binding methylated-DNA-[protein]-cysteine S-methyltransferase activity calcium ion binding methyltransferase activity DNA-methyltransferase activity catalytic activity transferase activity metal ion binding

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