Invitrogen

MGST1 Monoclonal Antibody (14H2)

MGST1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MGST1 Antibody (MA5-34942) in IHC (P)

Immunohistochemistry analysis of MGST1 in paraffin-embedded human liver carcinoma tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with MGST1 monoclonal antibody (Product # MA5-34942) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with... View More

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产品信息

MA5-34942

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:200

产品规格

种属反应

Human

已发表种属

Human, Insect

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

14H2

抗原

Recombinant protein within Human MGST1 aa 1-144

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_2848848

产品详细信息

Positive Control: SiHa cell, HepG2 cell, A549 cell, U937 cell, rat kidney tissue, human liver carcinoma tissue, human colon tissue, human placenta tissue, mouse liver tissue, MCF-7 cell.

靶标信息

The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Caveolin-1β promotes the production of active human microsomal glutathione S-transferase in induced intracellular vesicles inSpodoptera frugiperda21 insect cells.

Biochimica et biophysica acta. Biomembranes

Perrot N,Dessaux D,Rignani A,Gillet C,Orlowski S,Jamin N,Garrigos M,Jaxel C

MA5-34942 was used in Western Blot to show that caveolin-1β kept its capacity to induce intracellular vesicles in the host when co-expressed with hMGST1, and that hMGST1 is in part addressed to these vesicles.

Mon Aug 01 00:00:00 UTC 2022

The expression of Phase II drug-metabolizing enzymes in human B-lymphoblastoid TK6 cells.

Journal of environmental science and health. Part C, Toxicology and carcinogenesis

Li X,Li Y,Ning KG,Chen S,Guo L,Bonzo JA,Mei N

MA5-34942 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to develop a set of TK6-derived cell lines that individually express one of fourteen cytochrome P450s (CYPs).