Invitrogen

MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), APC, eBioscience™

MHC Class I (H-2Db) Antibody in Flow Cytometry (Flow)

图: 1 / 8

MHC Class I (H-2Db) Antibody (17-5999-82) in Flow

Staining of mouse splenocytes with 0.25 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (blue histogram) or 0.25 µg of Anti-Mouse MHC Class I (H-2Db) APC (purple histogram). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

17-5999-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看8篇已发表文章 8篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

28-14-8

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2573252

产品详细信息

Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.

Applications Reported: This 28-14-8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Lymph node colonization induces tumor-immune tolerance to promote distant metastasis.

Cell

Reticker-Flynn NE,Zhang W,Belk JA,Basto PA,Escalante NK,Pilarowski GOW,Bejnood A,Martins MM,Kenkel JA,Linde IL,Bagchi S,Yuan R,Chang S,Spitzer MH,Carmi Y,Cheng J,Tolentino LL,Choi O,Wu N,Kong CS,Gentles AJ,Sunwoo JB,Satpathy AT,Plevritis SK,Engleman EG

17-5999-82 was used in Flow cytometry/Cell sorting to show that an epigenetically instilled tumor-intrinsic interferon response program confers enhanced LN metastatic potential by enabling the evasion of NK cells and promoting LN colonization.

Thu May 26 00:00:00 UTC 2022

Autophagic Degradation of NBR1 Restricts Metastatic Outgrowth during Mammary Tumor Progression.

Developmental cell

Marsh T,Kenific CM,Suresh D,Gonzalez H,Shamir ER,Mei W,Tankka A,Leidal AM,Kalavacherla S,Woo K,Werb Z,Debnath J

17-5999 was used in Flow cytometry/Cell sorting to reveal that autophagy gene expression inversely correlates with pro-metastatic differentiation signatures and predicts overall and distant metastasis-free survival.

Mon Mar 09 00:00:00 UTC 2020

cGAS/STING axis mediates a topoisomerase II inhibitor-induced tumor immunogenicity.

The Journal of clinical investigation

Wang Z,Chen J,Hu J,Zhang H,Xu F,He W,Wang X,Li M,Lu W,Zeng G,Zhou P,Huang P,Chen S,Li W,Xia LP,Xia X

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 17-5999-82) in Flow Cytometry

Tue Aug 13 00:00:00 UTC 2019

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8⁺ T-cell responses by upregulating MHC-I expression.

Oncogene

Westrich JA,Vermeer DW,Silva A,Bonney S,Berger JN,Cicchini L,Greer RO,Song JI,Raben D,Slansky JE,Lee JH,Spanos WC,Pyeon D

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 17-5999-82) in Flow Cytometry

Fri Nov 01 00:00:00 UTC 2019

MULTI-seq: sample multiplexing for single-cell RNA sequencing using lipid-tagged indices.

Nature methods

McGinnis CS,Patterson DM,Winkler J,Conrad DN,Hein MY,Srivastava V,Hu JL,Murrow LM,Weissman JS,Werb Z,Chow ED,Gartner ZJ

17-5999-82 was used in Flow Cytometry to develop MULTI-seq: multiplexing using lipid-tagged indices for single-cell and single-nucleus RNA sequencing.

Mon Jul 01 00:00:00 UTC 2019

Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5.

The Journal of experimental medicine

Buxadé M,Huerga Encabo H,Riera-Borrull M,Quintana-Gallardo L,López-Cotarelo P,Tellechea M,Martínez-Martínez S,Redondo JM,Martín-Caballero J,Flores JM,Bosch E,Rodríguez-Fernández JL,Aramburu J,López-Rodríguez C

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 17-5999-82) in Flow Cytometry

Mon Nov 05 00:00:00 UTC 2018

The Shc1 adaptor simultaneously balances Stat1 and Stat3 activity to promote breast cancer immune suppression.

Nature communications

Ahn R,Sabourin V,Bolt AM,Hébert S,Totten S,De Jay N,Festa MC,Young YK,Im YK,Pawson T,Koromilas AE,Muller WJ,Mann KK,Kleinman CL,Ursini-Siegel J

Published figure using MHC Class I (H-2Db) monoclonal antibody (Product # 17-5999-82) in Flow Cytometry

Thu Mar 09 00:00:00 UTC 2017

Modulation of MHC class I surface expression in B16F10 melanoma cells by methylseleninic acid.

Oncoimmunology

Lennicke C,Rahn J,Bukur J,Hochgräfe F,Wessjohann LA,Lichtenfels R,Seliger B

17-5999 was used in Flow cytometry/Cell sorting to determine whether methylseleninic acid influences expression of MHC class I surface antigens in B16F10 melanoma cells.

Tue Jan 12 00:00:00 UTC 2021

生物信息学

蛋白别名: H-2 class I histocompatibility antigen, D-B alpha chain; H-2D cell surface glycoprotein; H-2D(B); H2-DC1-beta transplantation antigen protein; H2D1; H2DB; H2L; H2LD; MHC class I antigen; MHC class I H-2Dp; MHC class I H2 antigen; MHC H2-D-q alpha-chain; Q5k protein

查看更多收起

基因别名: H-2D; H2-D; H2-D1

查看更多收起

UniProt ID: (Mouse) P01899

查看更多收起

Entrez Gene ID: (Mouse) 14964

查看更多收起

功能