Invitrogen

MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), APC, eBioscience™

MHC Class I (H-2Kd) Antibody in Flow Cytometry (Flow)

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MHC Class I (H-2Kd) Antibody (17-5957-82) in Flow

Staining of C57BL/6 (left) or BALB/c (right) splenocytes with 0.06 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (blue histogram) or 0.06 µg of Anti-Mouse MHC Class I (H-2Kd) APC (purple histogram). Total viable cells were used for analysis.

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产品信息

17-5957-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

SF1-1.1.1

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1311276

产品详细信息

Description: This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, j, k, p, q, s, and v) has not been observed.

Applications Reported: This SF1-1.1.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SF1-1.1.1 antibody has been tested by flow cytometric analysis on Balb/c splenocytes. This antibody can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Disruption of the MSL complex inhibits tumour maintenance by exacerbating chromosomal instability.

Nature cell biology

Monserrat J,Morales Torres C,Richardson L,Wilson TS,Patel H,Domart MC,Horswell S,Song OR,Jiang M,Crawford M,Bui M,Dalal Y,Scaffidi P

17-5957-82 was used in Flow Cytometry to demonstrate that targeting MSL (male-specific lethal complex) may be a valuable means to increase CIN (chromosomal instability) beyond the level tolerated by cancer cells without inducing severe adverse effects in normal tissues.

Thu Apr 01 00:00:00 UTC 2021

Patient-derived xenograft culture-transplant system for investigation of human breast cancer metastasis.

Communications biology

Ma D,Hernandez GA,Lefebvre AEYT,Alshetaiwi H,Blake K,Dave KR,Rauf M,Williams JW,Davis RT,Evans KT,Longworth A,Masoud MYG,Lee R,Edwards RA,Digman MA,Kessenbrock K,Lawson DA

17-5957-82 was used in Flow cytometry/Cell sorting to demonstrate a new role for the metabolic enzyme NME1 in promoting breast cancer metastasis, providing proof-of-principle that our culture-transplant system can be used for authentic propagation and engineering of patient tumor cells for functional studies of metastasis.

Fri Nov 05 00:00:00 UTC 2021

Patient-derived xenograft culture-transplant system for investigation of human breast cancer metastasis.

Communications biology

Ma D,Hernandez GA,Lefebvre AEYT,Alshetaiwi H,Blake K,Dave KR,Rauf M,Williams JW,Davis RT,Evans KT,Longworth A,Masoud MYG,Lee R,Edwards RA,Digman MA,Kessenbrock K,Lawson DA

Fri Nov 05 00:00:00 UTC 2021

Multipotency of mouse trophoblast stem cells.

Stem cell research & therapy

Hou M,Han J,Li G,Kwon MY,Jiang J,Emani S,Taglauer ES,Park JA,Choi EB,Vodnala M,Fong YW,Emani SM,Rosas IO,Perrella MA,Liu X

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 17-5957-82) in Flow Cytometry

Thu Feb 13 00:00:00 UTC 2020

Targeting photodynamic and photothermal therapy to the endoplasmic reticulum enhances immunogenic cancer cell death.

Nature communications

Li W,Yang J,Luo L,Jiang M,Qin B,Yin H,Zhu C,Yuan X,Zhang J,Luo Z,Du Y,Li Q,Lou Y,Qiu Y,You J

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 17-5957-82) in Flow Cytometry

Fri Jul 26 00:00:00 UTC 2019

Interleukin-27 Is Essential for Type 1 Diabetes Development and Sjögren Syndrome-like Inflammation.

Cell reports

Ciecko AE,Foda B,Barr JY,Ramanathan S,Atkinson MA,Serreze DV,Geurts AM,Lieberman SM,Chen YG

17-5957-82 was used in Flow Cytometry to reveal that IL-27 contributes to autoimmunity in NOD mice through multiple mechanisms and provides substantial evidence to support its pathogenic role in human T1D.

Tue Dec 03 00:00:00 UTC 2019

Quiescent Tissue Stem Cells Evade Immune Surveillance.

Immunity

Agudo J,Park ES,Rose SA,Alibo E,Sweeney R,Dhainaut M,Kobayashi KS,Sachidanandam R,Baccarini A,Merad M,Brown BD

17-5957 was used in Flow cytometry/Cell sorting to identify a link between stem cell quiescence, antigen presentation, and immune evasion.

Tue Feb 20 00:00:00 UTC 2018

A therapeutic cancer vaccine against GL261 murine glioma.

Journal of translational medicine

Kindy MS,Yu J,Zhu H,Smith MT,Gattoni-Celli S

Published figure using MHC Class I (H-2Kd) monoclonal antibody (Product # 17-5957-82) in Flow Cytometry

Tue Jan 05 00:00:00 UTC 2016

Trastuzumab upregulates expression of HLA-ABC and T cell costimulatory molecules through engagement of natural killer cells and stimulation of IFNγ secretion.

Oncoimmunology

Chaganty BK,Lu Y,Qiu S,Somanchi SS,Lee DA,Fan Z

Fri Apr 01 00:00:00 UTC 2016

Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion.

European journal of immunology

Larsen J,Dall M,Antvorskov JC,Weile C,Engkilde K,Josefsen K,Buschard K

17-5957 was used in Flow cytometry/Cell sorting to study the in vitro and in vivo effects of gluten on natural killer cells.

Wed Oct 01 00:00:00 UTC 2014

Differential WNT activity in colorectal cancer confers limited tumorigenic potential and is regulated by MAPK signaling.

Cancer research

Horst D,Chen J,Morikawa T,Ogino S,Kirchner T,Shivdasani RA

17-5957 was used in Flow cytometry/Cell sorting to suggest that on its own, high WNT activity may not be a reliable signifier of tumor-initiating potential or stem-like potential.

Thu Mar 15 00:00:00 UTC 2012

ALDH1A isozymes are markers of human melanoma stem cells and potential therapeutic targets.

Stem cells (Dayton, Ohio)

Luo Y,Dallaglio K,Chen Y,Robinson WA,Robinson SE,McCarter MD,Wang J,Gonzalez R,Thompson DC,Norris DA,Roop DR,Vasiliou V,Fujita M

17-5957 was used in Flow cytometry/Cell sorting to demonstrate the existence of melanoma cells that fulfil the criteria for CSCs.

Mon Oct 01 00:00:00 UTC 2012

生物信息学

蛋白别名: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MGC184092; MGC7052; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

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基因别名: H-2K; H-2K(d); H2-K; H2-K1; K-f

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UniProt ID: (Mouse) P01901

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Entrez Gene ID: (Mouse) 14972

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功能