Invitrogen

MHC Class I (H-2Kd/H-2Dd) Monoclonal Antibody (34-1-2S), PE, eBioscience™

MHC Class I (H-2Kd/H-2Dd) Antibody in Flow Cytometry (Flow)

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MHC Class I (H-2Kd/H-2Dd) Antibody (12-5998-82) in Flow

Staining of BALB/c splenocytes with 0.125 µg of Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (blue histogram) or 0.125 µg of Anti-Mouse MHC Class I (H-2Kd/H-2Dd) PE (purple histogram). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-5998-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

34-1-2S

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_466122

产品详细信息

Description: The 34-1-2S monoclonal antibody reacts with the mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross-reacts with Kb, s, r, q, p.

Applications Reported: 34-1-2S has been reported for use in flow cytometric analysis.

Applications Tested: The 34-1-2S antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Pharmacologic inhibition of IL11/STAT3 signaling increases MHC-I expression and T cell infiltration.

Journal of translational medicine

Xiong W,Chen Y,Zhang C,Li J,Huang H,Zhu Y,Deng G,Cheng J,Lin Y,Shi Z,Mou T

Published figure using MHC Class I (H-2Kd/H-2Dd) monoclonal antibody (Product # 12-5998-82) in Flow Cytometry

Mon Jun 26 00:00:00 UTC 2023

Role of epidermal growth factor receptor inhibitor-induced interferon pathway signaling in the head and neck squamous cell carcinoma therapeutic response.

Journal of translational medicine

Korpela SP,Hinz TK,Oweida A,Kim J,Calhoun J,Ferris R,Nemenoff RA,Karam SD,Clambey ET,Heasley LE

12-5998-82 was used in Flow Cytometry to reveal heterogeneous, tumor cell-intrinsic, EGFR/ERBB inhibitor-induced IFN pathway activation in HNSCC and suggest that individual tumor responses to oncogene-targeted agents are a sum of direct growth inhibitory effects and variably-induced participation of host immune cells.

Sat Jan 23 00:00:00 UTC 2021

Phosphatase SHP1 impedes mesenchymal stromal cell immunosuppressive capacity modulated by JAK1/STAT3 and P38 signals.

Cell & bioscience

Jiang M,Ye J,Wang X,Li N,Wang Y,Shi Y

Published figure using MHC Class I (H-2Kd/H-2Dd) monoclonal antibody (Product # 12-5998-82) in Flow Cytometry

Thu Apr 14 00:00:00 UTC 2022

Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.

Journal for immunotherapy of cancer

Neuwelt AJ,Kimball AK,Johnson AM,Arnold BW,Bullock BL,Kaspar RE,Kleczko EK,Kwak JW,Wu MH,Heasley LE,Doebele RC,Li HY,Nemenoff RA,Clambey ET

Published figure using MHC Class I (H-2Kd/H-2Dd) monoclonal antibody (Product # 12-5998-82) in Flow Cytometry

Wed Apr 01 00:00:00 UTC 2020

Tissue-Resident Macrophages in Pancreatic Ductal Adenocarcinoma Originate from Embryonic Hematopoiesis and Promote Tumor Progression.

Immunity

Zhu Y,Herndon JM,Sojka DK,Kim KW,Knolhoff BL,Zuo C,Cullinan DR,Luo J,Bearden AR,Lavine KJ,Yokoyama WM,Hawkins WG,Fields RC,Randolph GJ,DeNardo DG

12-5998 was used in Flow cytometry/Cell sorting to uncover the heterogeneity of TAM origin and functions and could provide therapeutic insight for PDAC treatment.

Tue Aug 15 00:00:00 UTC 2017

Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy.

PloS one

Xiao GY,Liu IH,Cheng CC,Chang CC,Lee YH,Cheng WT,Wu SC

12-5998 was used in Flow cytometry/Cell sorting to demonstrate the therapeutic effects of AFSCs and suggest the use of AFSCs for treating infertility and POF.

Thu May 28 00:00:00 UTC 2015

Isolation and characterization of novel murine epiphysis derived mesenchymal stem cells.

PloS one

Cheng CC,Lian WS,Hsiao FS,Liu IH,Lin SP,Lee YH,Chang CC,Xiao GY,Huang HY,Cheng CF,Cheng WT,Wu SC

Published figure using MHC Class I (H-2Kd/H-2Dd) monoclonal antibody (Product # 12-5998-82) in Flow Cytometry

Fri Sep 07 00:00:00 UTC 2012

Antigen-specific immunity and cross-priming by epithelial ovarian carcinoma-induced CD11b(+)Gr-1(+) cells.

Journal of immunology (Baltimore, Md. : 1950)

Tomihara K,Guo M,Shin T,Sun X,Ludwig SM,Brumlik MJ,Zhang B,Curiel TJ,Shin T

12-5998 was used in Flow cytometry/Cell sorting to investigate CD11b(+)Gr-1(+) cells found in epithelial ovarian cancer-bearing mice, which are induced by ovarian cancer cells and undergo antigen-specific immunity and cross-priming.

Tue Jun 01 00:00:00 UTC 2010

Dynamics of lung macrophage activation in response to helminth infection.

Journal of leukocyte biology

Siracusa MC,Reece JJ,Urban JF,Scott AL

12-5998 was used in Flow cytometry/Cell sorting to study the dynamics of lung macrophage activation in response to helminth infection.

Mon Dec 01 00:00:00 UTC 2008

生物信息学

蛋白别名: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, D-B alpha chain; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2D cell surface glycoprotein; H-2D(B); H-2K(B); H-2K(K); H-2K(Q); H2-DC1-beta transplantation antigen protein; H2DD; H2K1; H2KD; MHC class I antigen; MHC class I H-2Dp; MHC class I H2 antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-D-q alpha-chain; MHC H2-K transplantation antigen; Q5k protein

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基因别名: H-2D; H-2K; H-2K(d); H2-D; H2-D1; H2-K; H2-K1; K-f

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UniProt ID: (Mouse) P01899, (Mouse) P01901

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Entrez Gene ID: (Mouse) 14964, (Mouse) 14972

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功能