Invitrogen

MHC Class II I-Ad Monoclonal Antibody (AMS-32.1), APC, eBioscience™

MHC Class II I-Ad Antibody in Flow Cytometry (Flow)

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MHC Class II I-Ad Antibody (17-5323-82) in Flow

Staining of C57Bl/6 (left) and Balb/c (right) splenocytes with 0.125 µg of Mouse IgG2b k Isotype Control APC (Product # 17-4732-81) (blue histogram) or 0.125 µg of Anti-Mouse MHC Class II I-Ad APC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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产品信息

17-5323-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

AMS-32.1

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10718531

产品详细信息

Description: This AMS-32.1 monoclonal antibody reacts with the mouse MHC Class II I-Ad alloantigen, which is present on mouse strains such as Balb/c and DBA/2. This antibody also recognizes the H-2f, H-2g7, H-2i, and H-2v haplotypes. However, reactivity to other haplotypes (e.g., b, k, p, q, r, s, and u) has not been observed.

Applications Reported: This AMS-32.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AMS-32.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Role of epidermal growth factor receptor inhibitor-induced interferon pathway signaling in the head and neck squamous cell carcinoma therapeutic response.

Journal of translational medicine

Korpela SP,Hinz TK,Oweida A,Kim J,Calhoun J,Ferris R,Nemenoff RA,Karam SD,Clambey ET,Heasley LE

17-5323-82 was used in Flow Cytometry to reveal heterogeneous, tumor cell-intrinsic, EGFR/ERBB inhibitor-induced IFN pathway activation in HNSCC and suggest that individual tumor responses to oncogene-targeted agents are a sum of direct growth inhibitory effects and variably-induced participation of host immune cells.

Sat Jan 23 00:00:00 UTC 2021

Effects of Combinatorial Ubiquitinated Protein-Based Nanovaccine and STING Agonist in Mice With Drug-Resistant and Metastatic Breast Cancer.

Frontiers in immunology

Huang F,Pan N,Wei Y,Zhao J,Aldarouish M,Wang X,Sun X,Wen Z,Chen Y,Wang L

17-5323-82 was used in Flow Cytometry to determine whether STING agonist enhances the anti-tumour activity of a protein-based nanovaccine.

Mon Dec 13 00:00:00 UTC 2021

Effects of Combinatorial Ubiquitinated Protein-Based Nanovaccine and STING Agonist in Mice With Drug-Resistant and Metastatic Breast Cancer.

Frontiers in immunology

Huang F,Pan N,Wei Y,Zhao J,Aldarouish M,Wang X,Sun X,Wen Z,Chen Y,Wang L

17-5323-82 was used in Flow Cytometry to determine whether STING agonist enhances the anti-tumour activity of a protein-based nanovaccine.

Mon Dec 13 00:00:00 UTC 2021

CD226 Deletion Reduces Type 1 Diabetes in the NOD Mouse by Impairing Thymocyte Development and Peripheral T Cell Activation.

Frontiers in immunology

Shapiro MR,Yeh WI,Longfield JR,Gallagher J,Infante CM,Wellford S,Posgai AL,Atkinson MA,Campbell-Thompson M,Lieberman SM,Serreze DV,Geurts AM,Chen YG,Brusko TM

Published figure using MHC Class II I-Ad monoclonal antibody (Product # 17-5323-82) in Flow Cytometry

Wed May 26 00:00:00 UTC 2021

Surface-modified P(HEMA-co-MAA) nanogel carriers for oral vaccine delivery: design, characterization, and in vitro targeting evaluation.

Biomacromolecules

Durán-Lobato M,Carrillo-Conde B,Khairandish Y,Peppas NA

17-5323 was used in Flow cytometry/Cell sorting to study how surface-modified P(HEMA-co-MAA) nanogel carriers could be a promising approach to a more efficacious oral vaccination regimen.

Mon Jul 14 00:00:00 UTC 2014

Recipient myeloid-derived immunomodulatory cells induce PD-1 ligand-dependent donor CD4+Foxp3+ regulatory T cell proliferation and donor-recipient immune tolerance after murine nonmyeloablative bone marrow transplantation.

Journal of immunology (Baltimore, Md. : 1950)

van der Merwe M,Abdelsamed HA,Seth A,Ong T,Vogel P,Pillai AB

17-5323 was used in Flow cytometry/Cell sorting to investigate the specific mechanisms of immune tolerance following TLI/ATS conditioning, showing the induction of PD-1 ligand-dependent donor Treg proliferation and donor-recipient immune tolerance.

Sun Dec 01 00:00:00 UTC 2013

生物信息学

蛋白别名: H-2 class II histocompatibility antigen, A beta chain; histocompatibility 2, class II antigen A, beta 1; major histocompatibility complex class II beta chain; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; response to metastatic cancers 1

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基因别名: Abeta; AI845868; H-2Ab; H2-Ab; H2-Ab1; H2-iabeta; I-Abeta; Ia-2; Ia2; IAb; Rmcs1

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UniProt ID: (Mouse) P14483

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Entrez Gene ID: (Mouse) 14961

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功能

protein binding transcription factor binding toxic substance binding ubiquitin protein ligase binding peptide antigen binding protein heterodimerization activity repressing transcription factor binding major histocompatibility complex protein

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