Invitrogen

MINA53 Monoclonal Antibody (M532)

MINA53 Antibody in Immunocytochemistry (ICC/IF)

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MINA53 Antibody (39-7300) in ICC/IF

Immunofluorescence analysis of MINA53 was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MINA53 (M532) Mouse Monoclonal Antibody (Product # 39-7300) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More

MINA53 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

39-7300

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

酶联免疫吸附实验 (ELISA)

Assay-dependent

染色质免疫沉淀实验 (ChIP)

查看4篇已发表文章 4篇已发表文章

竞争反应 (CA)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

M532

抗原

Recombinant full-length human Mina53

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533432

靶标信息

MINA is nuclear localized, myc-inducible protein that is thought to play a role in mammalian cell proliferation. Treatment of cancer cells lines such as the colon cancer cell line SW680 with siRNA against MINA inhibits cell growth, demonstrating that MINA may be a potential therapeutic target. MINA regulates several genes related to cell adhesion and metabolism that have also been shown to be regulated by c-Myc, but also regulates other genes whose expression are not modulated by c-Myc such as EGFR, IL-6 and HGF. MINA has also been found to act as a repressor to IL-4 expression in T cells, indicating that it may also play a role in T cell differentiation and genetic variation in T helper type 2 bias.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism.

Theranostics

Zhang Q,Wadgaonkar P,Xu L,Thakur C,Fu Y,Bi Z,Qiu Y,Almutairy B,Zhang W,Stemmer P,Chen F

39-7300 was used in Western Blot to suggest that mdig is a key mediator for the severity of COVID-19 in response to environmental exposure and targeting mdig may be the one of the effective strategies in ameliorating the symptom and reducing the mortality of COVID-19.

Fri Aug 06 00:00:00 UTC 2021

Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism.

Theranostics

Zhang Q,Wadgaonkar P,Xu L,Thakur C,Fu Y,Bi Z,Qiu Y,Almutairy B,Zhang W,Stemmer P,Chen F

Fri Aug 06 00:00:00 UTC 2021

Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism.

Theranostics

Zhang Q,Wadgaonkar P,Xu L,Thakur C,Fu Y,Bi Z,Qiu Y,Almutairy B,Zhang W,Stemmer P,Chen F

Fri Aug 06 00:00:00 UTC 2021

Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism.

Theranostics

Zhang Q,Wadgaonkar P,Xu L,Thakur C,Fu Y,Bi Z,Qiu Y,Almutairy B,Zhang W,Stemmer P,Chen F

Fri Aug 06 00:00:00 UTC 2021

ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.

Cancer research

Zhang L,Huo Q,Ge C,Zhao F,Zhou Q,Chen X,Tian H,Chen T,Xie H,Cui Y,Yao M,Li H,Li J

39-7300 was used in Western Blot to describe the mechanism by which zinc finger protein 143 promotes hepatocellular carcinoma proliferation and provide important clues for exploring new targets and strategies for clinical treatment of human liver cancer.

Mon Jun 15 00:00:00 UTC 2020

ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.

Cancer research

Zhang L,Huo Q,Ge C,Zhao F,Zhou Q,Chen X,Tian H,Chen T,Xie H,Cui Y,Yao M,Li H,Li J

Mon Jun 15 00:00:00 UTC 2020

CRISPR-Cas9 gene editing causes alternative splicing of the targeting mRNA.

Biochemical and biophysical research communications

Zhang Q,Fu Y,Thakur C,Bi Z,Wadgaonkar P,Qiu Y,Xu L,Rice M,Zhang W,Almutairy B,Chen F

39-7300 was used in Western Blot to conclude that cautions should be taken when using CRISPR-Cas9 strategy to edit human genes due to the unintended alterative splicing of the target mRNAs.

Sun Jul 12 00:00:00 UTC 2020

MINA53 deficiency leads to glioblastoma cell apoptosis via inducing DNA replication stress and diminishing DNA damage response.

Cell death & disease

Xuan F,Huang M,Zhao E,Cui H

39-7300 was used in Chromatin immunoprecipitation to determine the role of MYC-induced nuclear antigen in DNA replication initiation in glioblastoma cells.

Wed Oct 17 00:00:00 UTC 2018

MINA53 deficiency leads to glioblastoma cell apoptosis via inducing DNA replication stress and diminishing DNA damage response.

Cell death & disease

Xuan F,Huang M,Zhao E,Cui H

39-7300 was used in Chromatin immunoprecipitation to determine the role of MYC-induced nuclear antigen in DNA replication initiation in glioblastoma cells.

Wed Oct 17 00:00:00 UTC 2018

Transcriptional activation of Mina by Sp1/3 factors.

PloS one

Lian S,Potula HH,Pillai MR,Van Stry M,Koyanagi M,Chung L,Watanabe M,Bix M

39-7300 was used in western blot to characterize the Mina promoter.

Mon Sep 08 00:00:00 UTC 2014

Mina, an Il4 repressor, controls T helper type 2 bias.

Nature immunology

Okamoto M,Van Stry M,Chung L,Koyanagi M,Sun X,Suzuki Y,Ohara O,Kitamura H,Hijikata A,Kubo M,Bix M

39-7300 was used in ChIP assay and western blot to identify Mina as a genetic determinant of T helper type 2 bias

Sat Aug 01 00:00:00 UTC 2009

Increased expression of a Myc target gene Mina53 in human colon cancer.

The American journal of pathology

Teye K,Tsuneoka M,Arima N,Koda Y,Nakamura Y,Ueta Y,Shirouzu K,Kimura H

Thu Jan 01 00:00:00 UTC 2004

Increased expression of a Myc target gene Mina53 in human colon cancer.

The American journal of pathology

Teye K,Tsuneoka M,Arima N,Koda Y,Nakamura Y,Ueta Y,Shirouzu K,Kimura H

Thu Jan 01 00:00:00 UTC 2004

Increased expression of a Myc target gene Mina53 in human colon cancer.

The American journal of pathology

Teye K,Tsuneoka M,Arima N,Koda Y,Nakamura Y,Ueta Y,Shirouzu K,Kimura H

Thu Jan 01 00:00:00 UTC 2004

生物信息学

蛋白别名: 60S ribosomal protein L27a histidine hydroxylase; Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA; DKFZp762O1912; FLJ14393; Histone lysine demethylase MINA; MINA; mineral dust induced gene protein; Mineral dust-induced gene protein; MYC-induced nuclear antigen; myc-induced nuclear antigen, 53 kDa; Nucleolar protein 52; Protein mina53; Ribosomal oxygenase 2; Ribosomal oxygenase MINA; ROX

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基因别名: MDIG; MINA; MINA53; NO52; RIOX2; ROX

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UniProt ID: (Human) Q8IUF8

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Entrez Gene ID: (Human) 84864

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功能

transcriptional repressor activity, RNA polymerase II transcription factor binding iron ion binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors

参与通路

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated peptidyl-amino acid modification ribosome biogenesis post-translational protein modification negative regulation of transcription, DNA-templated oxidation-reduction process

MINA53 Monoclonal Antibody (M532)

MINA53 Antibody (39-7300) in ICC/IF

产品信息

39-7300

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

染色质免疫沉淀实验 (ChIP)

竞争反应 (CA)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

MINA53 Monoclonal Antibody (M532)

MINA53 Antibody (39-7300) in ICC/IF

产品信息

39-7300

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

染色质免疫沉淀 实验 (ChIP)

竞争反应 (CA)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)