Invitrogen

MKP-1 Recombinant Rabbit Monoclonal Antibody (JJ0930)

MKP-1 Antibody in Immunocytochemistry (ICC/IF)

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MKP-1 Antibody (MA5-32480) in ICC/IF

Immunocytochemistry analysis of MKP-1 with NIH/3T3 cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp), incubated with recombinant monoclonal MKP-1 (Product # MA5-32480) at a dilution of 1:100 (in 1% BSA in PBST overnight at 4°C), followed by Goat Anti-Rabbit IgG H&L 488 at 1:1,000 dilution (PBS used in control). Nuclear DNA was labelled in blue with DAPI, Beta tubulin (red) at 1/100 dilution... View More

MKP-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-32480

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化（石蜡） (IHC (P))

1:1,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JJ0930

抗原

Synthetic peptide within Human DUSP1 aa 112-156

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809757

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Tanshinone IIA protects motor neuron-like NSC-34 cells against lipopolysaccharide-induced cell injury by the regulation of the lncRNA TCTN2/miR-125a-5p/DUSP1 axis.

Regenerative therapy

Yan Q,Xun Y,Lei D,Zhai H

Published figure using MKP-1 recombinant monoclonal antibody (Product # MA5-32480) in Western Blot

Fri Dec 01 00:00:00 UTC 2023

Knockdown of TRIM9 attenuates irinotecan‑induced intestinal mucositis in IEC‑6 cells by regulating DUSP6 expression via the P38 pathway.

Molecular medicine reports

Zhao W,Wang Q

MA5-32480 was used in Western Blotting to investigate the effect of Tripartite motif family 9 on irinotecan‚induced intestinal mucositis in the rat intestinal epithelial cell line IEC‚6.

Wed Dec 01 00:00:00 UTC 2021

Knockdown of TRIM9 attenuates irinotecan‑induced intestinal mucositis in IEC‑6 cells by regulating DUSP6 expression via the P38 pathway.

Molecular medicine reports

Zhao W,Wang Q

MA5-32480 was used in Western Blotting to investigate the effect of Tripartite motif family 9 on irinotecan‚induced intestinal mucositis in the rat intestinal epithelial cell line IEC‚6.

Wed Dec 01 00:00:00 UTC 2021

生物信息学

蛋白别名: 3CH134/CL100 PTPase; CL 100; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1; EC 3.1.3.16; EC 3.1.3.48; MAP kinase phosphatase 1; MAP kinase phosphatase-1; mitogen-activated protein (MAP) kinase phosphatase-1; Mitogen-activated protein kinase phosphatase 1; mitogen-activated protein kinase phosphatase-1; oxidative stress-inducible protein tyrosine phosphatase; protein tyrosine phosphatase non-receptor type 16; protein tyrosine phosphatase, non-receptor type 16; Protein-tyrosine phosphatase 3CH134; Protein-tyrosine phosphatase CL100; Protein-tyrosine phosphatase ERP; Protein-tyrosine phosphatase non-receptor type 16; serine/threonine specific protein phosphatase

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基因别名: 3CH134; CL100; DUSP1; erp; HVH1; MKP-1; MKP1; PTPN10; Ptpn16; U19515; VH1

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UniProt ID: (Human) P28562, (Rat) Q64623, (Mouse) P28563

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Entrez Gene ID: (Human) 1843, (Rat) 114856, (Mouse) 19252

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