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MLH1 Antibody (MA5-32041) in ICC/IF
Immunofluorescence analysis of MLH1 was performed using 70% confluent log phase SW480 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. SW480 cells were labeled with MLH1 Rabbit Recombinant Monoclonal Antibody (Product # MA5-32041) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjug... View More
产品信息
MA5-32041
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SP08-04
抗原
Synthetic peptide within Human MLH1 aa 707-756
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809335
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: colon cancer, nonpolyposis type 2; DNA mismatch repair protein Mlh1; MGC5172; mismatch repair protein 1; MLH-1; mutL homolog 1, colon cancer, nonpolyposis type 2; MutL protein homolog 1
基因别名: 1110035C23Rik; AI317206; AI325952; AI561766; COCA2; FCC2; hMLH1; HNPCC; HNPCC2; MLH1
UniProt ID: (Human) P40692, (Rat) P97679, (Mouse) Q9JK91
Entrez Gene ID: (Human) 4292, (Rat) 81685, (Mouse) 17350
nuclear-transcribed mRNA poly(A) tail shortening
resolution of meiotic recombination intermediates
mismatch repair
double-strand break repair via nonhomologous end joining
male meiosis chromosome segregation
synapsis
spermatogenesis
intrinsic apoptotic signaling pathway in response to DNA damage
female meiosis chromosome segregation
somatic hypermutation of immunoglobulin genes
meiotic metaphase I plate congression
meiotic telomere clustering
isotype switching
negative regulation of mitotic recombination
oogenesis
meiotic spindle midzone assembly
response to hypoxia
somatic recombination of immunoglobulin genes involved in immune response
DNA repair
cellular response to DNA damage stimulus
meiotic nuclear division
reciprocal meiotic recombination
male meiosis
response to toxic substance
somatic recombination of immunoglobulin gene segments
response to drug
meiotic chromosome segregation
homologous chromosome segregation
cellular response to organic cyclic compound
cell cycle
