Invitrogen

MLKL Recombinant Rabbit Monoclonal Antibody (SA40-04)

MLKL Antibody in Immunocytochemistry (ICC/IF)

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MLKL Antibody (MA5-31971) in ICC/IF

Immunocytochemistry analysis of MLKL with HT-29 cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp), incubated with recombinant monoclonal MLKL (Product # MA5-31971) at a dilution of 1:100 (in 1% BSA in PBST overnight at 4°C), followed by Goat Anti-Rabbit IgG H&L 488 at 1:1,000 dilution (PBS used in control). Nuclear DNA was labelled in blue with DAPI, Beta tubulin (red) at 1/100 dilution (ov... View More

MLKL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MLKL Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with MLKL siRNA and reduction of signal was observed in Western Blot using MLKL Monoclonal Antibody (Product # MA5-31971). {KD}

产品信息

MA5-31971

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫组化（石蜡） (IHC (P))

1:50-1:2000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SA40-04

抗原

Recombinant protein within Human MLKL aa 333-471

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809265

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.