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MMP1 Antibody (PA5-27210) in ICC/IF
Immunofluorescence analysis of MMP1 was performed using 70% confluent log phase HUVEC and A549 untreated and Protein Transport Inhibitor (PTI) treated cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with MMP1 Polyclonal Antibody (Product # PA5-27210) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ R... View More
产品信息
PA5-27210
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 42 and 244 of Human MMP1
偶联物
形式
Liquid
浓度
0.54 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2544686
产品详细信息
Recommended positive controls: Raji, HUVEC, dermal fibroblast cells.
Predicted reactivity: Pig (90%), Rabbit (86%), Bovine (83%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Collagenase; collagenase-like A; Fibroblast collagenase; Interstitial collagenase; Interstitial collagenase A; matrix metallo protease; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloprotease 1; matrix metalloproteinase 1; matrix metalloproteinase 1a (interstitial collagenase); Matrix metalloproteinase-1; Matrix metalloproteinase-1a; Matrix metalloproteinase1; Mcol-A; MMP; MMP-1; MMP-1a; MMPs
基因别名: CLG; CLGN; Mcol-A; McolA; MMP1; Mmp1a
UniProt ID: (Human) P03956, (Mouse) Q9EPL5
Entrez Gene ID: (Human) 4312, (Mouse) 83995
