Invitrogen

MMP14 Recombinant Rabbit Monoclonal Antibody (SJ18-09)

MMP14 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

MMP14 Antibody (MA5-32076) in ICC/IF

Immunocytochemical analysis of MMP14 in BT-20 cells using a MMP14 Monoclonal antibody (Product # MA5-32076) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

MMP14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

产品信息

MA5-32076

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:10,000

免疫细胞化学 (ICC/IF)

1:50-1:200

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SJ18-09

抗原

Synthetic peptide within Human MMP14 aa 149-200

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809370

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

International journal of molecular sciences

Falkowski K,Bielecka E,Thøgersen IB,Bocheńska O,Płaza K,Kalińska M,Sąsiadek L,Magoch M,Pęcak A,Wiśniewska M,Gruba N,Wysocka M,Wojtysiak A,Brzezińska-Bodal M,Sychowska K,Pejkovska A,Rehders M,Butler G,Overall CM,Brix K,Dubin G,Lesner A,Kozik A,Enghild JJ,Potempa J,Kantyka T

MA5-32076 was used in Western Blotting to propose KLK14-mediated selective activation of cell-membrane located MT-MMPs as an additional layer of their regulation.

Fri Jun 19 00:00:00 UTC 2020

International journal of molecular sciences

MA5-32076 was used in Western Blotting to propose KLK14-mediated selective activation of cell-membrane located MT-MMPs as an additional layer of their regulation.

Fri Jun 19 00:00:00 UTC 2020

生物信息学

蛋白别名: EC 3.4.24.80; matrix metallo protease; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase 14 membrane-inserted; matrix metalloproteinase 14, membrane-inserted; Matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane type 1-matrix metalloproteinase; Membrane type 1-MMP; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP; MMP-14; MMP-X MT-MMP; MMP-X1; MMPs; MT-MMP; MT-MMP 1; MT-MMP MT1-MMP; MT1-MMP; MT1MMP; MTMMP1; type 1 matrix metalloprotease 14

查看更多收起

基因别名: AI325305; MMP-14; MMP-X1; MMP14; MT-MMP; MT-MMP 1; MT-MMP-1; MT1-MMP; MT1MMP; Mtmmp; MTMMP1; sabe; WNCHRS

查看更多收起

UniProt ID: (Human) P50281, (Mouse) P53690, (Rat) Q10739

查看更多收起

Entrez Gene ID: (Human) 4323, (Mouse) 17387, (Rat) 81707

查看更多收起

功能