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MMP3 Antibody (500-P324BT-25UG) in WB
Western Blot: To detect Human MMP-3 by Western Blot analysis, MMP3 Polyclonal Antibody, Biotin (Product # 500-P324BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human MMP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P324BT-25UG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human MMP-3
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930419
产品详细信息
AA Sequence of recombinant protein: MRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PETPLVPTEP VPPEPGTPAN CDPALSFDAV STLRGEILIF KDRHFWRKSL RKLEPELHLI SSFWPSLPSG VDAAYEVTSK DLVFIFKGNQ FWAIRGNEVR AGYPRGIHTL GFPPTVRKID AAISDKEKNK TYFFVEDKYW RFDEKRNSME PGFPKQIAED FPGIDSKIDA VFEEFGFFYF FTGSSQLEFD PNAKKVTHTL KSNSWLNC.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MMP-3. Anti-Human MMP-3-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human MMP-3 by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MMP-3 (500-P324) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human MMP-3.
Western Blot: To detect Human MMP-3 by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human MMP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P324BT-1MG will be provided as 2 x 500 µg
靶标信息
MMP3 (stromelysin-1) belongs to the peptidase M10A subfamily. It is secreted in its pro-protein form, and is activated by extracellular proteinase cleavage. MMP3 is capable of binding to zinc and calcium ions, and has metallo-endopeptidase activity. The active enzyme degrades collagen, gelatin, fibronectin, and laminin. It is involved in normal cellular processes such as wound repair and tissue remodeling. Mutations in the gene can result in coronary heart disease 6.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EC 3.4.24.17; matrix metallo protease; matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MMP; MMP-3; MMPs; proteoglycanase; SL-1; Stromelysin-1; Stromelysin1; Transin-1; Transin1
基因别名: CHDS6; MMP-3; MMP3; SL-1; STMY; STMY1; STR1
UniProt ID: (Human) P08254
Entrez Gene ID: (Human) 4314
