Invitrogen

MRAP2 Polyclonal Antibody

6个已发表图片

2 篇参考文献

查看其他9个MRAP2抗体

抗体检测数据 (4)
已发表图片 (6)

MRAP2 Antibody in Immunocytochemistry (ICC/IF)

MRAP2 Antibody in Immunocytochemistry (ICC/IF)

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MRAP2 Antibody (PA5-113283) in ICC/IF

Immunocytochemichal analysis of MRAP2 in U251 cells using a Polyclonal antibody (Product # PA5-113283) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.

MRAP2 Antibody in Immunocytochemistry (ICC/IF)

MRAP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MRAP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MRAP2 Antibody in Western Blot (WB)

MRAP2 Antibody in Western Blot (WB)

MRAP2 Antibody in Western Blot (WB)

MRAP2 Antibody in Western Blot (WB)

MRAP2 Antibody in Western Blot (WB)

MRAP2 Antibody in Western Blot (WB)

MRAP2 Antibody in Western Blot (WB)

Product Image Gallery

MRAP2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemichal analysis of MRAP2 in U251 cells using a Polyclonal antibody (Product # PA5-113283) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.

MRAP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunocytochemical analysis of paraffin-embeded MRAP2 in human brain tissue using a MRAP2 Polyclonal antibody (Product # PA5-113283) at a dilution of 1:100.

MRAP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunocytochemical analysis of paraffin-embeded MRAP2 in human lung tissue using a MRAP2 Polyclonal antibody (Product # PA5-113283) at a dilution of 1:100.

MRAP2 Antibody in Western Blot (WB) — Western Blot analysis of MRAP2 in multiple sample types using a MRAP2 polyclonal antibody (Product # PA5-113283) at a concentration of 7 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:10,000. Sample types that were tested include: Lane 1: Mouse brain tissue, Lane 2: Mouse kidney tissue.

MRAP2 Antibody in Western Blot (WB) — Analysis of PKR2 glycosylation in CHO cells in the presence of mouse MRAP2 and MRAP2 mutants. ( A ) Alignment of the human and mouse MRAP2 C-terminal domain. The sequence alignments of the region important for dimerization are shown in the box. ( B ) CHO stably expressing PKR2 untreated membrane proteins or those treated with PNGase F were analyzed by western blot analysis using an anti-PKR2 antibody. ( C ) Membrane proteins CHO stably expressing PKR2 were analyzed by western blot analysis using anti-PKR2 and anti-MRAP2 antibodies. In the absence of MRAP2, -; in the presence of mouse MRAP2, M; MRAP2 R125H mutant, R/H; MRAP2 R125C mutant, R/C.

MRAP2 Antibody in Western Blot (WB) — Interaction of MRAP2 isoforms with PKR2. ( A ) Membrane proteins from yeast co-expressing MRAP2 isoforms with PKR2 were co-precipitated using Ni-NTA His-bind resin and resolved by 12% SDS-PAGE. The immunoblots were probed with anti-His and anti-MRAP2 antibodies. Mouse MRAP2, M; human MRAP2, H; MRAP2 R125H, R/H; R125C MRAP2, R/C. ( B ) GST pull-down experiments. The GST fusion protein of PKR2-NT was used to pull down MRAP2 isoforms. Mouse MRAP2, M; R125H mutant, R/H; R125C mutant, R/C. Input and solutions obtained by elution were analyzed by western blotting with anti-GST and anti-MRAP2. I, input; E, eluate.

MRAP2 Antibody in Western Blot (WB) — Analysis of MRAP2 regions that bind PKR2. ( A ) Scheme of human MRAP2 gene. Exon coding sequences are indicated as black bars and the untranslated sequences are shown as white bars. ( B ) In the box, the amino acid sequence of the C-terminal MRAP2 domain; the sequence present in the 131CT-MRAP2 domain is indicated in red. ( C ) SDS PAGE and Western blot analysis of CT-MRAP2 domain expression in E. coli ; lane 1: 5 mug; lane 2: 10 mug. ( D ) Western blot analysis of native PAGE of CT-MRAP2 domain expression in E. coli ; lane 1: 10 mug; lane 2: 15 mug. ( E ) The GST fusion protein and PKR2-NT were used to pull-down the CT-MRAP2 domain. Solutions obtained by elution were analyzed by Western blotting: lane 1; PKR2-NT eluate; lane 2: GST eluate. ( F ) The GST fusion protein and PKR2-NT were used to pull-down the 131CT-MRAP2 domain. Solutions obtained by elution were analyzed by Western blotting: lane 1; PKR2-NT eluate; lane 2: GST eluate.

MRAP2 Antibody in Western Blot (WB) — Interaction of MRAP2 with the N-terminal region of PKR2. ( A ) Membranes from yeast co-expressing MRAP2 with PKR2 or DeltaN-PKR2 were co-precipitated using Ni-NTA His-bind resin and were resolved by 12% SDS-PAGE. The immunoblots were probed with anti-His (left panel) and anti-MRAP2 (right panel) antibodies. Lane 1: Cy12946, lane 2: DeltaN-PKR2, and lane 3: PKR2. ( B ) The GST fusion protein PKR2-NT was used to pull-down MRAP2, and the elution solutions obtained were resolved 15% SDS-PAGE analyzed by Western blotting using an anti-MRAP2 antibody. Lane 1: input; lane 2: PKR2-NT eluate; and lane 3: GST eluate.

MRAP2 Antibody in Western Blot (WB) — Figure 5 MRAP2 expression in adipocytes and hypothalamic explants. MRAP2 levels in adipose tissue were analyzed by ( A ) real-time RT-PCR and ( B ) Western blot analysis. MRAP2 levels in hypothalamus explants analyzed by ( C ) real-time RT-PCR and ( D ) Western blot analysis (arrow). Data are expressed as the mean +- SEM of three separate experiments (n = 4 per group). Statistical analyses were performed using Student's t -test where ** p < 0.01; *** p < 0.001 vs. WT ( A , B ) and one-way ANOVA followed by Tukey's post-test, * p < 0.05 ( C , D ).

MRAP2 Antibody in Western Blot (WB) — Cross-linking of MRAP2 with PKR2 or DeltaN-PKR2 receptors. Membrane and cytosolic proteins prepared from S. cerevisiae cells expressing the PKR2-MRAP2 and DeltaN-PKR2 -MRAP2 were incubated in the presence (+) or absence (-) of BS 3 . Proteins were immunoblotted and probed with an anti-MRAP2 antibody. The arrow indicates the complex PKR2/MRAP2.

产品信息

PA5-113283

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Melanocortin-2 receptor accessory protein 2 protein (66-205AA)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

AB_2868017

靶标信息

Modulator of melanocortin receptor 4 (MC4R), a receptor involved in energy homeostasis. Plays a central role in the control of energy homeostasis and body weight regulation by increasing ligand-sensitivity of MC4R and MC4R-mediated generation of cAMP. May also act as a negative regulator of MC2R: competes with MRAP for binding to MC2R and impairs the binding of corticotropin (ACTH) to MC2R. May also regulate activity of other melanocortin receptors (MC1R, MC3R and MC5R); however, additional evidences are required in vivo.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Arginine 125 Is an Essential Residue for the Function of MRAP2.

International journal of molecular sciences

Fullone MR,Maftei D,Vincenzi M,Lattanzi R,Miele R

PA5-113283 was used in Western Blotting to evaluate the role of Arginine 125 of MRAP2 in mouse.

Tue Aug 30 00:00:00 UTC 2022

Arginine 125 Is an Essential Residue for the Function of MRAP2.

International journal of molecular sciences

Fullone MR,Maftei D,Vincenzi M,Lattanzi R,Miele R

PA5-113283 was used in Western Blotting to evaluate the role of Arginine 125 of MRAP2 in mouse.

Tue Aug 30 00:00:00 UTC 2022

Identification of Regions Involved in the Physical Interaction between Melanocortin Receptor Accessory Protein 2 and Prokineticin Receptor 2.

Biomolecules

Fullone MR,Maftei D,Vincenzi M,Lattanzi R,Miele R

Published figure using MRAP2 polyclonal antibody (Product # PA5-113283) in Western Blot

Sun Mar 20 00:00:00 UTC 2022

生物信息学

蛋白别名: MC2R accessory protein 2; Melanocortin-2 receptor accessory protein 2

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基因别名: bA51G5.2; BB633055; BMIQ18; C6orf117; MRAP2

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UniProt ID: (Human) Q8IXM9, (Mouse) D3Z1Q2

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Entrez Gene ID: (Human) 112609, (Mouse) 244958

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protein binding corticotropin hormone receptor binding type 3 melanocortin receptor binding type 4 melanocortin receptor binding type 5 melanocortin receptor binding identical protein binding type 1 melanocortin receptor binding

energy reserve metabolic process feeding behavior positive regulation of cAMP biosynthetic process protein localization to cell surface energy homeostasis