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MRPL19 Antibody (PA5-115590) in ICC/IF
Immunocytochemistry analysis of MRPL19 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115590) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115590
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MRPL19(Accession P49406), corresponding to amino acid residues Y197-L247.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900225
产品详细信息
Antibody detects endogenous levels of total MRPL19.
靶标信息
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 39S ribosomal protein L15, mitochondrial; 39S ribosomal protein L19, mitochondrial; L15mt; L19mt; Large ribosomal subunit protein bL19m; MRP-L19; ribosomal protein, mitochondrial, L15
基因别名: 9030416F12Rik; 9130412E02Rik; D6Ertd157e; KIAA0104; L19mt; MRP-L15; MRP-L19; MRPL15; MRPL19; RLX1; RPML15
UniProt ID: (Human) P49406, (Mouse) Q9D338
Entrez Gene ID: (Human) 9801, (Mouse) 56284, (Rat) 297372
