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MRPL28 Antibody (MA5-42510) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of MRPL28 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with MRPL28 Recombinant Monoclonal Antibody (Product # MA5-42510) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blu... View More
产品信息
MA5-42510
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat,
Zebrafish
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JE55-90
抗原
Synthetic peptide within N-terminal Human MRPL28
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2911651
产品详细信息
Positive Control: Mouse liver tissue lysate, SW480 cell lysate, rat stomach tissue lysate, human skin tissue lysate, A549 cell lysate, K562 cell lysate, Hela cell lysate, zebrafish tissue lysates, 293T, MCF-7, SW480, human tonsil tissue, human liver tissue, human liver carcinoma tissue.
Subcellular Location: Mitochondrion.
靶标信息
MRP-L28 (39S ribosomal protein L28, melanoma-associated antigen recognized by T lymphocytes) is a mitochondrial protein that belongs to the ribosomal protein L28P family. MRP-L28 potentially represents an important therapeutic reagent for HLA-A24 (A24) patients as this antigen is recognized by tumor-infiltrating lymphocyte (TIL) 1290, which targets the A24 serotype. HLAA24 (A24) is an HLA-A serotype that identifies the more common HLA-A24 gene products. A24 is a split antigen that is also recognized by the A9 broad antigen type and the similar A23 types. A24 is common in Austronesia and has one of the highest A allele frequencies for a number of peoples, including Papua New Guineans, indigeonous Taiwanese (eastern tribals), Yupik and Greenland Eskimos. MRP-L28 is found in a variety of normal tissues including spleen, testis, thymus, liver, kidney, brain, adrenal, lung and retinal tissue.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 39S ribosomal protein L28, mitochondrial; HGNC6756; L28mt; Large ribosomal subunit protein bL28m; MAAT 1; Melanoma antigen p15; melanoma-associated antigen recognised by cytotoxic T lymphocytes; melanoma-associated antigen recognized by T lymphocytes; Melanoma-associated antigen recognized by T-lymphocytes; MGC8499; mitochondrial ribosomal protein L28; MRP L28; MRP-L28; MRPL 28
基因别名: 1110015G04Rik; L28mt; MAAT1; MRP-L28; MRPL28; p15; wu:fa96b11; zgc:110013
UniProt ID: (Human) Q13084, (Mouse) Q9D1B9
Entrez Gene ID: (Human) 10573, (Rat) 497876, (Mouse) 68611, (Zebrafish) 554098
