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MRPL28 Antibody (MA5-42510) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of MRPL28 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with MRPL28 Recombinant Monoclonal Antibody (Product # MA5-42510) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blu... View More
Product Details
MA5-42510
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE55-90
Immunogen
Synthetic peptide within N-terminal Human MRPL28
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2911651
Product Specific Information
Positive Control: Mouse liver tissue lysate, SW480 cell lysate, rat stomach tissue lysate, human skin tissue lysate, A549 cell lysate, K562 cell lysate, Hela cell lysate, zebrafish tissue lysates, 293T, MCF-7, SW480, human tonsil tissue, human liver tissue, human liver carcinoma tissue.
Subcellular Location: Mitochondrion.
Target Information
MRP-L28 (39S ribosomal protein L28, melanoma-associated antigen recognized by T lymphocytes) is a mitochondrial protein that belongs to the ribosomal protein L28P family. MRP-L28 potentially represents an important therapeutic reagent for HLA-A24 (A24) patients as this antigen is recognized by tumor-infiltrating lymphocyte (TIL) 1290, which targets the A24 serotype. HLAA24 (A24) is an HLA-A serotype that identifies the more common HLA-A24 gene products. A24 is a split antigen that is also recognized by the A9 broad antigen type and the similar A23 types. A24 is common in Austronesia and has one of the highest A allele frequencies for a number of peoples, including Papua New Guineans, indigeonous Taiwanese (eastern tribals), Yupik and Greenland Eskimos. MRP-L28 is found in a variety of normal tissues including spleen, testis, thymus, liver, kidney, brain, adrenal, lung and retinal tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 39S ribosomal protein L28, mitochondrial; HGNC6756; L28mt; Large ribosomal subunit protein bL28m; MAAT 1; Melanoma antigen p15; melanoma-associated antigen recognised by cytotoxic T lymphocytes; melanoma-associated antigen recognized by T lymphocytes; Melanoma-associated antigen recognized by T-lymphocytes; MGC8499; mitochondrial ribosomal protein L28; MRP L28; MRP-L28; MRPL 28
Gene Aliases: 1110015G04Rik; L28mt; MAAT1; MRP-L28; MRPL28; p15; wu:fa96b11; zgc:110013
UniProt ID: (Human) Q13084, (Mouse) Q9D1B9
Entrez Gene ID: (Human) 10573, (Rat) 497876, (Mouse) 68611, (Zebrafish) 554098
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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