Invitrogen

MSRA Polyclonal Antibody

MSRA Antibody in Immunocytochemistry (ICC/IF)

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MSRA Antibody (PA5-88562) in ICC/IF

Immunofluorescence analysis of MSRA in NIH/3T3 cells. Samples were incubated with MSRA Polyclonal antibody (Product # PA5-88562) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

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产品信息

PA5-88562

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 24-235 of human MSRA (NP_0364631)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.21 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2804997

产品详细信息

Immunogen sequence: GNSASNIVSP QEALPGRKEQ TPVAAKHHVN GNRTVEPFPE GTQMAVFGMG CFWGAERKFW VLKGVYSTQV GFAGGYTSNP TYKEVCSEKT GHAEVVRVVY QPEHMSFEEL LKVFWENHDP TQGMRQGNDH GTQYRSAIYP TSAKQMEAAL SSKENYQKVL SEHGFGPITT DIREGQTFYY AEDYHQQYLS KNPNGYCGLG GTGVSCPVGI KK; Positive Samples: Mouse kidney, Rat kidney; Cellular Location: Cytoplasm, Lipid-anchor, Membrane, Mitochondrion, Nucleus

靶标信息

Methionine sulfoxide reductase (MsrA) reduces methionine sulfoxide (MetO) residues in proteins and free MetO to Methionine (Met). The catalytic activity of MsrA is dependent of bound metal and cofactors but it requires reducing equivalents from either DTT or a thioredoxin-regenerating system. MsrA plays an essential role in protecting cells against oxidative damage. The substrates of MsrA include calmodulin, HIV protease and a 1-proteinase-inhibitor. Recent studies indicate that there is a connection between MsrA and Alzheimer's disease in mammals.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Methionine oxidation activates pyruvate kinase M2 to promote pancreatic cancer metastasis.

Molecular cell

He D,Feng H,Sundberg B,Yang J,Powers J,Christian AH,Wilkinson JE,Monnin C,Avizonis D,Thomas CJ,Friedman RA,Kluger MD,Hollingsworth MA,Grandgenett PM,Klute KA,Toste FD,Chang CJ,Chio IIC

PA5-88562 was used in Western Blot to demonstrate that methionine residues can act as reversible redox switches governing distinct signaling outcomes and that the MSRA-PKM2 axis serves as a regulatory nexus between redox biology and cancer metabolism to control tumor metastasis.

Thu Aug 18 00:00:00 UTC 2022

生物信息学

蛋白别名: cytosolic methionine-S-sulfoxide reductase; Mitochondrial peptide methionine sulfoxide reductase; peptide met (O) reductase; Peptide Met(O) reductase; peptide methionine sulfoxide reductase; Peptide-methionine (S)-S-oxide reductase; PMSR; Protein-methionine-S-oxide reductase

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基因别名: 2310045J23Rik; 6530413P12Rik; MSR-A; MSRA; PMSR

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UniProt ID: (Human) Q9UJ68, (Rat) Q923M1, (Mouse) Q9D6Y7

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Entrez Gene ID: (Human) 4482, (Rat) 29447, (Mouse) 110265

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功能

peptide-methionine (S)-S-oxide reductase activity oxidoreductase activity oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor reductase

参与通路

cellular protein modification process methionine metabolic process response to oxidative stress protein repair oxidation-reduction process aging