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MST1 (STK4) Antibody (PA5-22015) in ICC/IF
Immunofluorescence analysis of MST1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MST1 Polyclonal Antibody (Product # PA5-22015) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnigh... View More
产品信息
PA5-22015
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to a region within amino acids 324 and 418 of STK4 (Uniprot ID#Q13043)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_11157025
产品详细信息
Recommended positive controls: HepG2, mouse brain.
Predicted reactivity: Mouse (100%), Rat (100%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
STK4 (MST1) is a serine/threonine kinase which may be involved in apoptosis. It is possible that this protein induces the chromatin condensation observed in this process. STK4 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: EC 2.7.11.1; kinase MST1; kinase responsive to stress 2; Mammalian STE20-like protein kinase 1; mammalian sterile 20-like 1; MST-1; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; TIIAC
基因别名: KRS2; MST1; STK4; YSK3
UniProt ID: (Human) Q13043
Entrez Gene ID: (Human) 6789
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
protein binding
ATP binding
transcription factor binding
identical protein binding
protein homodimerization activity
protein serine/threonine kinase activator activity
protein dimerization activity
non-receptor serine/threonine protein kinase
cell morphogenesis
patterning of blood vessels
neural tube formation
positive regulation of protein phosphorylation
endocardium development
protein phosphorylation
apoptotic process
signal transduction
central nervous system development
negative regulation of cell proliferation
peptidyl-serine phosphorylation
signal transduction by protein phosphorylation
keratinocyte differentiation
positive regulation of protein binding
positive regulation of peptidyl-serine phosphorylation
hippo signaling
intracellular signal transduction
positive regulation of apoptotic process
positive regulation of fat cell differentiation
negative regulation of organ growth
protein autophosphorylation
protein stabilization
primitive hemopoiesis
cell differentiation involved in embryonic placenta development
regulation of cell differentiation involved in embryonic placenta development
positive regulation of protein serine/threonine kinase activity
negative regulation of canonical Wnt signaling pathway
hepatocyte apoptotic process
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
