Invitrogen

MTCO2 Monoclonal Antibody (4B12A5)

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MTCO2 Antibody (459150) in WB

The Journal of cell biology 2022 - Figure S4. mtDNA damage results in mitochondrial dysfunction. (A) Representative images of cells expressing Cox4::GFP stained with TMRE after treatment with FCCP (2 muM) for 20 min or Oligomycin (0.5 mug/ml) for 4 h (left panel). Total TMRE intensity normalized to cell area is plotted; n > 650 pooled from three biological replicates for FCCP and Oligomycin and two biological replicates for empty vector (rig... View More

Product Image Gallery

MTCO2 Antibody — Figure 6 Comparison of mitochondrial parameters in wild-type and puf3Delta mutant strains treated with the TORC1 inhibitor rapamycin. (A) Mitochondrial oxygen consumption of cells treated with rapamycin during early log phase and analyzed during late log phase. (B) Western blot analysis of protein extracts derived from late log phase cultures (representative of three biological replicates). (C) Steady-state levels of COX17 , COX12 , and RIP1 transcripts in late log phase. The values indicate the mean +/- SD with p-values indicated as described in the legend of Figure 1 . {TM}

产品信息

459150

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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产品规格

种属反应

Yeast

已发表种属

Yeast

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

4B12A5

抗原

Full length purified native protein

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

IgG fraction

保存液

HEPES buffered saline

内含物

0.02% sodium azide

保存条件

4° C, do not freeze

运输条件

Wet ice

AB_2532228

产品详细信息

Positive control: Mitochondria from yeast membrane extract

靶标信息

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Pbp1 associates with Puf3 and promotes translation of its target mRNAs involved in mitochondrial biogenesis.

PLoS genetics

van de Poll F,Sutter BM,Acoba MG,Caballero D,Jahangiri S,Yang YS,Lee CD,Tu BP

459150 was used in Western Blot to reveal a key role for Pbp1-containing assemblies in enabling the translation of mRNAs critical for mitochondrial biogenesis and respiration.

Mon May 01 00:00:00 UTC 2023

DarT-mediated mtDNA damage induces dynamic reorganization and selective segregation of mitochondria.

The Journal of cell biology

Dua N,Seshadri A,Badrinarayanan A

459150 was used in Western Blot to suggest that, under damage, partitioning of defective mtDNA and organelle are de-coupled, with emphasis on mitochondrial segregation independent of its DNA.

Mon Oct 03 00:00:00 UTC 2022

Mapping the Saccharomyces cerevisiae Spatial Proteome with High Resolution Using hyperLOPIT.

Methods in molecular biology (Clifton, N.J.)

Nightingale DJH,Oliver SG,Lilley KS

459150 was used in Western Blotting to describe a protocol for the unbiased and high-throughput study of protein subcellular localization in Saccharomyces cerevisiae via hyperLOPIT.