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MTM1 Antibody (PA5-89279) in WB
Western blot analysis of MTM1 in extracts of Mouse thymus. Samples were incubated with MTM1 Polyclonal antibody (Product # PA5-89279) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-89279
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 484-603 of human MTM1 (NP_0002431)
偶联物
形式
Liquid
浓度
1.14 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805464
产品详细信息
Immunogen sequence: SARERQKVTE RTVSLWSLIN SNKEKFKNPF YTKEINRVLY PVASMRHLEL WVNYYIRWNP RIKQQQPNPV EQRYMELLAL RDEYIKRLEE LQLANSAKLS DPPTSPSSPS QMMPHVQTHF; Positive Samples: Mouse thymus; Cellular Location: Cell membrane, Cell projection, Cytoplasm, Late endosome, Peripheral membrane protein, filopodium, ruffle
靶标信息
Human MTM1, a 603 amino-acid protein, is mutated in myotubular myopathy. The largely related protein hMTMR2 is found mutated in a recessive form of Charcot-Marie-tooth neuropathy. Myotubularin is primarily a lipid phosphatase that acts on hosphatidylinositol 3-monophosphate and is involved in the regulation of the phosphatidylinositol 3-kinase (PI 3-kinase) pathway and membrane trafficking. Wildtype myotubularin can directly dephosphorylate PI 3-P and PI 4-P in vitro. Thus, it decreases PI 3-P levels by down-regulating PI 3-K activity and by facilitating the degradation of PI 3-P.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Myotubularin; Phosphatidylinositol-3,5-bisphosphate 3-phosphatase; Phosphatidylinositol-3-phosphate phosphatase; X-linked myotubular myopathy gene 1
基因别名: AF073996; mKIAA4176; Mtm; Mtm1
UniProt ID: (Rat) Q6AXQ4, (Mouse) Q9Z2C5
Entrez Gene ID: (Rat) 288762, (Mouse) 17772
molecular_function
phosphatidylinositol-3-phosphatase activity
phosphoprotein phosphatase activity
protein tyrosine phosphatase activity
intermediate filament binding
phosphatidylinositol binding
phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity
protein binding
hydrolase activity
phosphatase activity
phosphatase
protein dephosphorylation
biological_process
endosome to lysosome transport
protein transport
peptidyl-tyrosine dephosphorylation
regulation of vacuole organization
intermediate filament organization
muscle cell cellular homeostasis
phosphatidylinositol dephosphorylation
mitochondrion distribution
mitochondrion morphogenesis
lipid metabolic process
transport
dephosphorylation
